Membrane-bound (MUC1) and secretory (MUC2, MUC3, and MUC4) mucin gene expression in human lung cancer
Membrane-bound (MUC1) and secretory (MUC2, MUC3, and MUC4) mucin gene expression in human lung cancer
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DOI：
10.1159/000217980
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发表时间：
1996-05-01
期刊：
影响因子：
--
通讯作者：
Ho, SB
中科院分区：
文献类型：
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作者：
Nguyen, PL;Niehans, GA;Ho, SB
粘蛋白型糖蛋白在肺癌中的异常已被描述，但其分子基础尚不清楚。本研究利用粘蛋白核心肽抗体和cDNA探针，检测了正常肺(非肿瘤性)和肺肿瘤组织中与一种膜结合粘蛋白(MUC1)、两种肠道粘蛋白(MUC2和MUC3)和一种气管支气管壁粘蛋白(MUC4)相对应的粘蛋白基因的相对表达。正常肺组织表现出明显的粘蛋白基因表达模式，高水平表达MUC1和MUC4mRNA，低水平或不表达MUC2和MUC3粘蛋白免疫反应和mRNA。相反，肺腺癌，尤其是高分化癌，表现出MUC1、MUC3和MUC4的mRNA水平升高。肺鳞状细胞癌、腺鳞状细胞癌和大细胞癌的特征仅是MUC4粘蛋白水平升高。我们的结论是，在肺肿瘤中，一种膜结合型粘蛋白和几种分泌型粘蛋白的表达是独立调节的，并发生了明显的变化。在各种非小细胞肺癌中，MUC4转录本的频繁出现表明了这种类型的粘蛋白在肺癌生物学中的潜在重要性。
Abnormalities of mucin-type glycoproteins have been described in lung cancers, but their molecular basis is unknown. In this study, mucin-core-peptide-specific antibodies and cDNA probes were used to determine the relative expression of mucin genes corresponding to one membrane-bound mucin (MUC1), two intestinal mucins (MUC2 and MUC3), and one tracheobronchial mucin (MUC4) in normal (nonneoplastic) lung, and in lung neoplasms. Normal lung tissues exhibited a distinct pattern of mucin gene expression, with high levels of MUC1 and MUC4 mRNA and low to absent levels of MUC2 and MUC3 mucin immunoreactivity and mRNA. In contrast, lung adenocarcinomas, especially well-differentiated cancers, exhibited increased MUC1, MUC3, and MUC4 mRNA levels. Lung squamous-cell, adenosquamous, and large-cell carcinomas were characterized by increased levels of MUC4 mucin only. We conclude that the expression of one membrane-bound and several secretory-type mucins is independently regulated and markedly altered in lung neoplasms. The frequent occurrence of increased MUC4 transcripts in a variety of non-small-cell lung cancers indicates the potential importance of this type of mucin in lung cancer biology.