Characterization of the Escherichia coli σE regulon
Characterization of the Escherichia coli σE regulon
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DOI：
10.1074/jbc.m100464200
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发表时间：
2001-06-15
影响因子：
4.8
通讯作者：
Raina, S
中科院分区：
文献类型：
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作者：
Dartigalongue, C;Missiakas, D;Raina, S
大肠杆菌通过诱导热休克基因的转录来响应错误折叠蛋白的积累。E sigma（E）RNA聚合酶控制两个热休克调节子之一的关闭。这种调节子在双膜被膜中错误折叠的多肽积累时被激活。杆菌sigma（E）（RpoE）是sigma因子的细胞质外功能亚家族的成员。在这里，我们问有多少基因被E sigma（E）RNA聚合酶激活，这些基因的身份是什么。采用两种独立的遗传学方法，对20株E.鉴定了以σ（E）依赖性方式激活报道基因转录的大肠杆菌启动子。在检查的所有情况下，在映射转录起始位点时可以揭示典型的sigma（E）结合位点。10个鉴定的启动子激活了先前鉴定的基因的转录，其中4个基因直接作用于折叠。大肠杆菌包膜蛋白（dsbC、fkpA、skp和surA），其余的启动子转录被推测编码迄今未知的胞质外功能的基因，并被命名为ecf（ecfA-ecfM）。其中两个ecf基因是大肠杆菌所必需的。大肠杆菌生长。
Escherichia coli responds to the accumulation of misfolded proteins by inducing the transcription of heat shock genes. E sigma (E) RNA polymerase controls one of the two heat shock regulons off. coli, This regulon is activated upon accumulation of misfolded polypeptides in the double membrane envelope off. coli. sigma (E) (RpoE) is a member of the extracytoplasmic function subfamily of sigma factors. Here we asked how many genes are activated by E sigma (E) RNA polymerase and what is the identity of these genes. Using two independent genetic approaches, 20 E. coli promoters were identified which activate reporter gene transcription in a sigma (E)-dependent manner. In all cases examined, a canonical sigma (E) binding site could be revealed upon mapping transcriptional start sites. 10 identified promoters activated the transcription of previously identified genes with four genes acting directly on the folding off. coli envelope proteins (dsbC, fkpA, skp, and surA), The remaining promoters transcribed genes that are presumed to encode hitherto unknown extracytoplasmic functions and were named ecf (ecfA-ecfM). Two of these ecf genes were found to be essential for E. coli growth.