Bulky DNA adducts in human sperm: relationship with fertility, semen quality, smoking, and environmental factors
Bulky DNA adducts in human sperm: relationship with fertility, semen quality, smoking, and environmental factors
复制标题
DOI：
10.1016/s1383-5718(03)00051-2
复制
发表时间：
2003-05-09
影响因子：
1.9
通讯作者：
Widlak, P
中科院分区：
文献类型：
--
作者：
Horak, S;Polanska, J;Widlak, P
生精细胞的DNA完整性会受到内源性和外源性基因毒性因素的影响。由此导致的精子DNA损伤可能对生育能力受损有重大影响。在此，采用P - 32后标记法对179名男性（包括健康供体和生育能力受损的患者）的精子细胞中大块DNA加合物的水平进行了分析。对所有供体进行分析时，吸烟者的大块DNA加合物水平比非吸烟者高1.2倍，但差异无统计学意义（P = 0.054）。然而，在健康个体中，当前吸烟者和从不吸烟者之间存在统计学上的显著差异（增加1.7倍，P = 0.008）。未发现饮酒或咖啡摄入与精子DNA加合物之间存在相关性。精子中DNA加合物的水平似乎不受环境和职业因素的影响。另一方面，健康人群组和男性因素不育患者组在大块DNA加合物水平方面存在显著差异（P = 0.012）。在生育能力受损的患者中，DNA加合物与精子浓度或精子活力之间存在显著的负相关（n = 93；P < 0.029，rₛ = - 0.225）。这些结果表明，精子细胞中的DNA加合物可作为人类不育研究中的潜在生物标志物。（C）2003爱思唯尔科学出版社。保留所有权利。
The integrity of DNA of spermatogenic cells can be affected by endogenous and exogenous genotoxic factors. Resulting DNA damage in spermatozoa may significantly contribute to impaired fertility. Here, the P-32-postlabeling method was used to analyze the levels of bulky DNA adducts in sperm cells in a group of 179 males, either healthy donors or patients with an impaired fertility. When all donors were analyzed, the levels of bulky DNA adducts was 1.2-fold higher in smokers than in non-smokers, but the difference was not statistically significant (P = 0.054). However, a statistically significant difference existed between current smokers and never smokers among the healthy individuals (1.7-fold increase, P = 0.008). No correlation between alcohol or coffee consumption and sperm DNA adducts was found. The levels of DNA adducts in sperm seemed to be unaffected by environmental and occupational factors. On the other hand, groups of healthy persons and patients with male-factor infertility differed significantly with respect to the level of bulky DNA adducts (P = 0.012). A significant negative correlation between DNA adducts and sperm concentration or sperm motility existed among patients with an impaired fertility (it = 93; P < 0.029, rs = -0.225). These results suggest that DNA adducts in sperm cells can be applied as potential biomarkers in studies of human infertility. (C) 2003 Elsevier Science B.V. All rights reserved.