POSTMORTEM CHANGES IN HIGH-AFFINITY CHOLINE UPTAKE
POSTMORTEM CHANGES IN HIGH-AFFINITY CHOLINE UPTAKE
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DOI：
10.1111/j.1471-4159.1979.tb11089.x
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发表时间：
1979-01-01
影响因子：
4.7
通讯作者：
KUHAR, MJ
中科院分区：
文献类型：
--
作者：
KLEMM, N;KUHAR, MJ
When animals were killed by decapitation and the heads kept refrigerated at 2-4.degree. C, high affinity choline uptake was maintained up to 3 days post-mortem. At 5 days post-mortem, there was a significant reduction in uptake. In tissues kept at 2-4.degree. C for 1 day, the ionic dependence, drug sensitivity and kinetic parameters of uptake were identical to that of control tissues. At 3 days post-mortem, intact synaptosomal profiles although with features characteristic of degenerating neuronal tissues, were observed in EM studies. In tissue maintained at room temperature, the uptake activity was nearly completely gone by 1 day. High affinity choline uptake is maintained for days, a surprisingly long time, in tissues kept in the cold immediately after death. When pentylenetetrazol or pentobarbital were administered to rats to activate or depress the choline uptake, the activity-related changes in choline uptake undergo a reversal in post-mortem tissues. The changes in uptake were significantly lost by 10 min post-mortem and totally absent by 30 min post-mortem. In vitro studies with whole hippocampi indicate that the post-mortem reversal in activity-related changes in uptake is temperature-dependent. Because of post-mortem reversals in activity-related states of uptake, the true magnitude of these activity-related changes in uptake may be underestimated by existing methods of assay. Acetylcholine levels in synaptosomal preparations did not clearly correlate with levels of choline uptake.
