From cells to muropeptide structures in 24 h: peptidoglycan mapping by UPLC-MS.
From cells to muropeptide structures in 24 h: peptidoglycan mapping by UPLC-MS.
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DOI：
10.1038/srep07494
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发表时间：
2014-12-16
影响因子：
4.6
通讯作者：
Bertsche U
中科院分区：
文献类型：
--
作者：
Kühner D;Stahl M;Demircioglu DD;Bertsche U
肽聚糖（PGN）在几乎所有细菌物种中普遍存在。PGN球囊保护细胞免受其自身内部膨压的影响，使PGN成为抗菌治疗的最重要靶点之一。在过去的60年里，人们用各种方法对PGN的组成进行了深入的研究。突破性进展是HPLC技术在多肽分析中的应用。然而，纯PGN的制备依赖于约一周的非常耗时的方法。我们建立了革兰氏阳性菌和革兰氏阴性菌的纯化方案，该方案可以在塑料反应管中完全进行，在24小时内产生纯的尿肽。 可以通过UPLC-MS分析所述胞肽，从而允许其立即测定。这种新的快速方法提供了即使在高通量下筛选PGN组合物的可行性，使其成为基础研究以及制药工业的非常有用的工具。
Peptidoglycan (PGN) is ubiquitous in nearly all bacterial species. The PGN sacculus protects the cells against their own internal turgor making PGN one of the most important targets for antibacterial treatment. Within the last sixty years PGN composition has been intensively studied by various methods. The breakthrough was the application of HPLC technology on the analysis of muropeptides. However, preparation of pure PGN relied on a very time consuming method of about one week. We established a purification protocol for both Gram-positive and Gram-negative bacteria which can be completely performed in plastic reaction tubes yielding pure muropeptides within 24 hours. The muropeptides can be analyzed by UPLC-MS, allowing their immediate determination. This new rapid method provides the feasibility to screen PGN composition even in high throughput, making it a highly useful tool for basic research as well as for the pharmaceutical industry.
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影响因子：
16.6
作者：
Pilhofer, Martin;Aistleitner, Karin;Biboy, Jacob;Gray, Joe;Kuru, Erkin;Hall, Edward;Brun, Yves V.;VanNieuwenhze, Michael S.;Vollmer, Waldemar;Horn, Matthias;Jensen, Grant J.
通讯作者：
Jensen, Grant J.
影响因子：
3.7
作者：
Bertsche, Ute;Yang, Soo-Jin;Weidenmaier, Christopher
通讯作者：
Weidenmaier, Christopher
影响因子：
3.7
作者：
Takacs, Constantin N.;Hocking, Jason;Jacobs-Wagner, Christine
通讯作者：
Jacobs-Wagner, Christine
影响因子：
4.9
作者：
Baek, Kristoffer T.;Grundling, Angelika;Frees, Dorte
通讯作者：
Frees, Dorte
DOI：
10.3791/51183
发表时间：
2014-01-15
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Desmarais SM;Cava F;de Pedro MA;Huang KC
通讯作者：
Huang KC