Detachment of Microtubules Driven by Kinesin Motors from Track Surfaces Under External Force
Detachment of Microtubules Driven by Kinesin Motors from Track Surfaces Under External Force
复制标题
驱动蛋白马达驱动的微管在外力作用下从轨道表面脱离
DOI：
10.1007/978-3-030-92163-7_16
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Nitta Takahiro
中科院分区：
文献类型：
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作者：
Sweet May;Nitta Takahiro
马达蛋白，如肌球蛋白和动蛋白，是生物分子马达，参与活细胞中力的产生和物质的运输。马达蛋白及其相关的细胞骨架细丝，如肌动蛋白细丝和微管，已被用于工程系统的主动运输。在控制主动输运时，通常使用通过电场或流体流动的外力。使用外力的一个缺点是，外力会导致微管从滑动表面脱落。脱离导致货物丢失或微管表面密度稀疏，从而限制了外力的可获得性。应该把超脱降到最低。在这样做的时候，对脱离过程的详细观察将是有帮助的。然而，由于其空间和时间分辨率的限制，实验研究受到阻碍。在这里，我们展示了一个外力作用下微管在涂有动蛋白马达的表面滑行的模拟研究。由于计算机模拟具有较高的空间和时间分辨率，发现了两种脱离模式。揭示了两种模式的具体过程，对减少脱体有一定的参考价值。
Motor proteins, such as myosin and kinesin, are biological molecular motors involved in force generation and material transport in living cells. Motor proteins and their associated cytoskeletal filaments, such as actin filaments and microtubules, have been utilized for active transport in engineered systems. In controlling the active transport, external forces via electric fields or fluid flow were commonly used. A drawback of using external force is that the external force can cause detachment of microtubules from gliding surfaces. Detachment leads to loss of cargo or sparse surface density of microtubules, thus limiting the availability of external forces. Detachment should be minimized. In doing so, detailed observation on the process of detachment would be helpful. However, due to its limited spatial and temporal resolution, experimental investigations are hampered. Here, we show a simulation study for the detachment of microtubules gliding over surfaces coated with kinesin motors by an external force. Owing to the computer simulation’s high spatial and time resolution, two modes of detachment were found. Detailed processes of the two modes were revealed, which would be useful to diminish detachment.