Genetic basis of sRNA quantitative variation analyzed using an experimental population derived from an elite rice hybrid.
Genetic basis of sRNA quantitative variation analyzed using an experimental population derived from an elite rice hybrid.
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使用源自优良水稻杂交种的实验群体分析 sRNA 数量变异的遗传基础
DOI：
10.7554/elife.03913
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发表时间：
2015-03-30
期刊：
影响因子：
7.7
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Wang J;Yao W;Zhu D;Xie W;Zhang Q
我们对水稻永生化F2（IMF 2）群体剑叶中sRNA丰度进行了遗传分析。我们鉴定了53，613，739个独特的sRNA和165，797个sRNA表达性状（s-traits）。共检测到66，649个s性状，定位了40，049个本地sQTL和30，809个远缘sQTL。通过定义80，362个sRNA簇，为所有50，139个sRNA簇表达性状（sc-traits）中的20，249个恢复了22，263个sRNA簇QTL（scQTL）。来自相同基因或相同sRNA簇的大多数s-性状的表达水平呈轻微正相关。虽然对于一部分sRNA观察到来自相同母亲基因的sRNA之间以及sRNA与其母亲基因之间的遗传共调节，但大多数sRNA及其母亲基因几乎没有显示出共调节。一些sRNA生物发生基因位于远距离sQTL热点区域，并与特定长度的sRNA相对应，表明它们在sRNA的调控和生物发生中起着重要作用。DOI：http://dx.doi.org/10.7554/eLife.03913.001网站
We performed a genetic analysis of sRNA abundance in flag leaf from an immortalized F2 (IMF2) population in rice. We identified 53,613,739 unique sRNAs and 165,797 sRNA expression traits (s-traits). A total of 66,649 s-traits mapped 40,049 local-sQTLs and 30,809 distant-sQTLs. By defining 80,362 sRNA clusters, 22,263 sRNA cluster QTLs (scQTLs) were recovered for 20,249 of all the 50,139 sRNA cluster expression traits (sc-traits). The expression levels for most of s-traits from the same genes or the same sRNA clusters were slightly positively correlated. While genetic co-regulation between sRNAs from the same mother genes and between sRNAs and their mother genes was observed for a portion of the sRNAs, most of the sRNAs and their mother genes showed little co-regulation. Some sRNA biogenesis genes were located in distant-sQTL hotspots and showed correspondence with specific length classes of sRNAs suggesting their important roles in the regulation and biogenesis of the sRNAs. DOI: http://dx.doi.org/10.7554/eLife.03913.001