Highly sensitive and interference-free simultaneous detection of two polycyclic aromatic hydrocarbons at parts-per-trillion levels using a surface plasmon resonance immunosensor
Highly sensitive and interference-free simultaneous detection of two polycyclic aromatic hydrocarbons at parts-per-trillion levels using a surface plasmon resonance immunosensor
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DOI：
10.1016/j.snb.2004.04.076
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发表时间：
2004-09-29
影响因子：
8.4
通讯作者：
Miura, N
中科院分区：
文献类型：
--
作者：
Gobi, KV;Miura, N
基于表面等离子体共振（SPR）技术，研制了一种新型的流动免疫传感器，用于同时检测潜在的内分泌干扰物苯并（a）芘（BaP）和致癌物2-羟基联苯（HBP）。SPR免疫传感器芯片采用易成型的柔性聚二甲基硅氧烷（PDMS）薄膜制成。通过模压工艺制备了具有多微通道结构的PDMS薄膜，并将其用作流动池组件。随着多微通道流动池系统的使用，小至300 RI的样品足以同时对混合分析物溶液中存在的两种不同化合物进行SPR分析。该SPR免疫传感器与间接竞争免疫反应方法配合使用，可在ppt（parts-per-trillon）水平上检测低分子量抗原BaP和HBP。针对BaP和HBP的单克隆抗体在存在于分析物溶液中的抗原BaP和HBP与固定在SPR传感器芯片上的抗原-蛋白质缀合物之间进行竞争性免疫反应。SPR响应的免疫反应，以确定在混合的分析物溶液中的BaP和HBP的浓度。SPR免疫传感器被证明可以同时检测BaP和HBP，分别在10 ppt-300 ppb和10 ppt-1000 ppb的浓度范围内，响应时间约为15 min。SPR免疫传感器显示出两种分析物之间的交叉灵敏度可以忽略不计。使用胃蛋白酶作为洗脱液，实现了用于重复使用的传感器芯片的再生。(C)2004 Elsevier B. V.保留所有权利。
A novel surface plasmon resonance (SPR)-based flow immunosensor is fabricated for simultaneous detection of benzo(a)pyrene (BaP), a potential endocrine disrupter, and 2-hydroxybiphenyl (HBP), a carcinogen. Immunosensor chip for SPR imaging is fabricated using easily moldable, flexible, thin polydimethylsiloxane (PDMS) films. PDMS films having multi-microchannel structures are prepared by molding process and act as flow-cell module. With the use of multi-microchannel flow-cell systems, samples of as small as 300 RI are enough for SPR analysis of two different compounds present in a mixed analyte solution simultaneously. The SPR immunosensor working with indirect competitive immunoreaction method is demonstrated to detect the low molecular weight antigens, BaP and HBP, at ppt (parts-per-triIlion) levels. Monoclonal antibodies against BaP and HBP undergo competitive immunoreaction between the antigens, BaP and HBP, present in analyte solution and the antigen-protein conjugates immobilized on the SPR sensor chip. The SPR responses of the immunoreaction are followed to determine the concentration of BaP and HBP in mixed analyte solutions. The SPR immumosensor is demonstrated to detect BaP and HBP at the same time in the concentration range of 10 ppt-300 ppb and 10 ppt-1000 ppb, respectively, with a response time of approximately 15 min. The SPR immunosensor shows negligible cross-sensitivity between the two analytes. Regeneration of the sensor chip for repeated use is attained with the use of pepsin as the eluent. (C) 2004 Elsevier B.V. All rights reserved.