Antibiotic susceptibility patterns for environmental streptococci isolated from bovine mastitis in central California dairies
Antibiotic susceptibility patterns for environmental streptococci isolated from bovine mastitis in central California dairies
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DOI：
10.3168/jds.s0022-0302(02)74061-7
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发表时间：
2002-01-01
影响因子：
3.5
通讯作者：
Cullor, JS
中科院分区：
文献类型：
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作者：
Rossitto, PV;Ruiz, L;Cullor, JS
环境链球菌经常从奶牛乳房炎中分离出来，但关于这些生物体的抗菌药物敏感性的信息有限。在研究中使用了从加州圣华金河谷中部的牛乳房炎病例中分离的总共362株环境链球菌。总体而言，39.9%的测试菌株是链球菌，停乳链球菌42.2%，肠球菌属11.1%。测定了这些微生物对以下抗菌剂的抗菌药物敏感性：青霉素、氨苄青霉素、头孢噻吩、头孢噻呋、青霉素+新生霉素、红霉素、吡利霉素、四环素和磺胺二甲氧嘧啶。结果表明，统称为环境链球菌的各种生物体的敏感性模式有很大的差异。青霉素对152株沙门氏菌的MIC 90为0.06 μ g/ml。停乳，而133株S.不稳定。然而，肠球菌属（Enterococcus spp.）是测试的耐药性最强的微生物。这些数据还表明，使用基于人类数据的解释标准可能会产生误导性结果。总之，这些数据证实，环境链球菌是由几个不同的属和种组成的一组不同的生物体，需要在种水平上鉴定环境链球菌，以适当修改控制方法。此外，禁忌使用琼脂纸片扩散（Kirby-Bauer）药敏试验检测具有人源性判读标准的病原体，这些试验仅应使用具有乳腺炎特异性判读标准的病原体进行。
Environmental streptococci are frequently isolated from bovine mastitis in dairy cows with only limited information available on the antimicrobial susceptibility of these organisms. A total of 362 environmental streptococci isolated from cases of bovine mastitis from the central San Joaquin Valley of California over a 3-yr period were used in the study. Overall, 39.9% of the strains tested were Streptococcus uberis, 42.2% were Streptococcus dysgalactiae, and 11.1% were Enterococcus spp. The antimicrobial susceptibility for these organisms was determined for the following antimicrobial agents: penicillin, ampicillin, cephalothin, ceftiofur, penicillin + novobiocin, erythromycin, pirlimycin, tetracycline, and sulfadimethoxine. Results demonstrate substantial differences in the susceptibility patterns for the various organisms collectively referred to as the environmental streptococci. The MIC90 for penicillin was 0.06 mug/ml for 152 strains of S. dysgalactiae compared with 0.25 mug/ml for 133 strains of S. uberis. However, the Enterococcus spp. were the most resistant organisms tested. These data also indicate that the use of interpretive criteria based on human data may provide misleading results. In conclusion, these data confirm that the environmental streptococci are a diverse group of organisms comprised of several different genera and species and that identification of environmental streptococci to the species level is needed to appropriately modify control methods. Moreover, the use of the agar disk diffusion (Kirby-Bauer) susceptibility test for agents with human-based interpretive criteria is contraindicated, and these tests should only be performed with agents with mastitis specific interpretive criteria.