EXPERIMENTAL REORGANIZATION OF CEREBELLAR CORTEX .1. MORPHOLOGICAL EFFECTS OF ELIMINATION OF ALL MICRONEURONS WITH PROLONGED X-IRRADIATION STARTED AT BIRTH
EXPERIMENTAL REORGANIZATION OF CEREBELLAR CORTEX .1. MORPHOLOGICAL EFFECTS OF ELIMINATION OF ALL MICRONEURONS WITH PROLONGED X-IRRADIATION STARTED AT BIRTH
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DOI：
10.1002/cne.901460305
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发表时间：
1972-01-01
影响因子：
2.5
通讯作者：
ANDERSON, WJ
中科院分区：
文献类型：
--
作者：
ALTMAN, J;ANDERSON, WJ
Long-Evans大鼠的头部从出生时开始接受低水平X射线重复剂量的照射，以防止获得出生后形成的篮状细胞、星状细胞和颗粒细胞。这些微神经元的缺乏导致了小脑皮质组织的几个变化，在30日龄时用光学显微镜和电子显微镜进行了研究。浦肯野细胞胞体不呈单层排列。它们的初级树突是随机定向的，并且根据它们的胞体在皮层深处的位置而不同地分支。许多通常只在婴儿中看到的浦肯野细胞体周突起被保留下来。攀缘纤维和苔藓纤维与胞体形成不明显的对称突触，与浦肯野细胞的体周突起和树突刺形成明显的不对称突触。大量的浦肯野细胞树突和无数的刺构成了大脑皮层的大部分神经元。树突棘，除了形成真正的突触，还建立了假突触与胶质细胞的过程和突触没有囊泡的表面或内部的浦肯野细胞树突。后一种类型的接触显然导致部分树突的自溶，这是观察到的唯一明确的病理现象。这些结果表明，浦肯野细胞树突的生长具有相当大的自主性，在缺乏平行纤维的情况下，树突丰富地分支并萌发出无数的小突起，并与不适当的突起（如浦肯野细胞或星形胶质细胞的突起）邻接形成突触后致密膜。
The heads of Long‐Evans rats were irradiated from birth with a schedule of repeated doses of low‐level x‐ray which prevented the acquisition of the postnatally‐forming basket, stellate and granule cells. The absence of these microneurons led to several changes in the organization of the cerebellar cortex, studied at 30 days of age with light and electron microscopy. The somata of Purkinje cells were not strung out in a monolayer. Their primary dendrites were randomly oriented and arborized differently depending on the position of their somata in the depth of the cortex. Many Purkinje cell perisomatic processes, normally seen only in infants, were retained. Climbing and mossy fibers formed inconspicuous, symmetrical synapses with the somata and conspicuous, asymmetrical synapses with the perisomatic processes and dendritic thorns of Purkinje cells. Massive Purkinje cell dendrites with innumerable thorns made up much of the neuropil of the cortex. The dendritic thorns, in addition to forming true synapses, also established pseudosynapses with glial processes and synapses devoid of vesicles with the surface or the interior of Purkinje cell dendrites. The latter type of contact apparently led to autolysis of part of the dendrite, which was the only clear pathological phenomenon observed. These results indicated considerable autonomy in the growth of Purkinje cell dendrites, which arborized richly and sprouted innumerable small processes in the absence of parallel fibers, and formed postsynaptic dense membranes in contiguity with inappropriate processes, such as those of Purkinje cells or astrocytes.