Dynamic changes of microtubule and actin structures in CV-1 cells during electrofusion.
Dynamic changes of microtubule and actin structures in CV-1 cells during electrofusion.
复制标题
电融合过程中CV-1细胞微管和肌动蛋白结构的动态变化。
DOI：
10.1002/cm.970170409
复制
发表时间：
1990
影响因子：
--
通讯作者：
Chang,DC
中科院分区：
文献类型：
--
作者：
Zheng,QA;Chang,DC
为了研究细胞骨架系统在动物细胞融合中的作用，我们检测了细胞融合不同阶段细胞骨架蛋白的动态变化。应用射频电脉冲进行CV-L细胞的融合。用双标记荧光显微镜同时观察微管和F-肌动蛋白的结构变化。观察到，在细胞融合开始后的几分钟内，MT束开始延伸到通过融合相邻细胞膜而形成的细胞质桥中。后来，在融合细胞的相邻核之间出现了平行的MT束网络；这种MT束可能提供了负责核聚集的机械连接。Factin在细胞融合过程中的结构变化更为复杂。我们观察到融合细胞中肌动蛋白分布的许多不同模式，包括一些巨大的环状结构。肌动蛋白的重组不太可能参与核聚集过程。相反，在细胞边缘凝聚的肌动蛋白束可能有助于加宽细胞质桥梁，从而允许融合细胞之间的细胞内容合并。
To study the involvement of the cytoskeletal system in the fusion of animal cells, we examined the dynamic changes of cytoskeletal proteins during the various stages of cell fusion. CV‐l cells were fused by applying a radio‐frequency electrical pulse. Structural changes of microtubules (MTs) and F‐actin were monitored simultaneously by double‐label fluorescence microscopy. It was observed that in a few minutes after the initiation of cell fusion, MT bundles began to extend into the cytoplasmic bridges which were formed by fusing the membranes of neighboring cells. Later, a network of parallel MT bundles appeared between the adjacent nuclei of the fusing cells; such MT bundles may provide the mechanical links that are responsible for nuclear aggregation. The structural changes of Factin during cell fusion were more complicated. We observed many different patterns of actin distribution in the fusing cells, including some giant, ring‐shaped structures. Reorganization of actin is unlikely to be involved in the nuclear aggregation process. Instead, actin bundles condensed at the cell edges may help to widen the cytoplasmic bridges to allow merging of cellular contents between the fusing cells.