Thermal properties of poly(3-hydroxy-2-methylbutyrate-co-3-hydroxybutyrate) copolymers with narrow comonomer-unit compositional distributions
Thermal properties of poly(3-hydroxy-2-methylbutyrate-co-3-hydroxybutyrate) copolymers with narrow comonomer-unit compositional distributions
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DOI：
10.1038/s41428-021-00545-4
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发表时间：
2021-08-12
期刊：
影响因子：
2.8
通讯作者：
Tsuge, Takeharu
中科院分区：
文献类型：
--
作者：
Furutate, Sho;Abe, Hideki;Tsuge, Takeharu
利用重组大肠杆菌LSBJ生物合成了（2 R，3R）-3-羟基-2-甲基丁酸酯（3 H2 MB）和（R）-3-羟基丁酸酯（3 HB）[P（3 H2 MB-co-3 HB）]与不同共聚单体组成的新型聚羟基链烷酸酯（PHA）共聚物。此外，进行生物合成P（24摩尔% 3 H2 MB-co-3 HB）的溶剂分馏以获得具有窄共聚单体单元组成分布的共聚物。P（3 H2 MB-co-3 HB）的熔融温度（T-m）随共聚单体含量的变化表明共聚物具有同二型性。P（3 H2 MB）与3 HB共聚后仍保持快速结晶行为。P（80 mol% 3 H2 MB-co-3 HB）共聚物的T-m接近P（3 HB）的T-m，表现出比P（3 HB）均聚物更快的结晶行为。因此，3 H2 MB和3 HB的共聚对于设计具有期望性能的新PHA材料是有价值的。
Novel polyhydroxyalkanoate (PHA) copolymers of (2R, 3R)-3-hydroxy-2-methylbutyrate (3H2MB) and (R)-3-hydroxybutyrate (3HB) [P(3H2MB-co-3HB)] with various comonomer compositions were biosynthesized using recombinant Escherichia coli LSBJ. Furthermore, solvent fractionation of biosynthesized P(24 mol% 3H2MB-co-3HB) was performed to obtain copolymers with narrow comonomer-unit compositional distributions. The melting temperature (T-m) of P(3H2MB-co-3HB) as a function of the comonomer content indicated isodimorphism of the copolymer. The fast crystallization behavior of P(3H2MB) was maintained even when it was copolymerized with 3HB. The P(80 mol% 3H2MB-co-3HB) copolymer, with a T-m close to that of P(3HB), exhibited faster crystallization behavior than the P(3HB) homopolymer. Therefore, copolymerization of 3H2MB and 3HB is valuable for the design of new PHA materials with desirable properties.