TRANSLOCATION OF CAMPYLOBACTER-JEJUNI ACROSS HUMAN POLARIZED EPITHELIAL-CELL MONOLAYER-CULTURES
TRANSLOCATION OF CAMPYLOBACTER-JEJUNI ACROSS HUMAN POLARIZED EPITHELIAL-CELL MONOLAYER-CULTURES
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DOI：
10.1093/infdis/166.2.308
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发表时间：
1992-08-01
影响因子：
6.4
通讯作者：
CIEPLAK, W
中科院分区：
文献类型：
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作者：
KONKEL, ME;MEAD, DJ;CIEPLAK, W
通过使用在微孔滤膜上生长的极化 Caco-2 细胞单层来研究空肠弯曲杆菌分离株跨上皮细胞屏障易位的能力。测试的 4 个空肠弯曲菌分离株均穿过 Caco-2 细胞单层并表现出相似的易位动力学。穿过极化细胞单层的细菌数量随着时间继续增加，直到接种后 4 小时，此时观察到最大易位率。透射电子显微镜显示，空肠弯曲菌通过穿过细胞和在细胞之间穿过极化的 Caco-2 细胞单层。氯霉素是一种细菌蛋白质合成抑制剂，可减少空肠弯曲菌的易位。低温下细菌的附着、内化和易位受到抑制。这些数据表明空肠弯曲菌的粘附、渗透和易位需要活跃的细菌和靶细胞过程，并进一步表明细胞易位在空肠弯曲菌介导的肠炎的发病机制中的作用。
The ability of Campylobacter jejuni isolates to translocate across an epithelial cell barrier was investigated by using polarized Caco-2 cell monolayers grown on microporous membrane filters. The 4 C.jejuni isolates tested all traversed the Caco-2 cell monolayers and displayed similar translocation kinetics. The number of bacteria crossing the polarized cell monolayers continued to increase with time until 4 h after inoculation, at which time a maximum rate of translocation was observed. Transmission electron microscopy revealed that C.jejuni translocated across polarized Caco-2 cell monolayers by passing both through and between cells. Chloramphenicol, an inhibitor of bacterial protein synthesis, reduced the translocation of C.jejuni. Bacterial attachment, internalization, and translocation were inhibited at low temperature. These data indicate that adherence, penetration, and translocation of C.jejuni require active bacterial and target cell processes and further suggest a role for cellular translocation in the pathogenesis of C.jejuni-mediated enteritis.