Molecular phylogenetic diversity of the emerging mucoralean fungus Apophysomyces: Proposal of three new species
Molecular phylogenetic diversity of the emerging mucoralean fungus Apophysomyces: Proposal of three new species
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DOI：
10.1016/j.riam.2010.01.006
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发表时间：
2010-06-30
影响因子：
1.9
通讯作者：
Guarro, Josep
中科院分区：
文献类型：
--
作者：
Alvarez, Eduardo;Stchigel, Alberto M.;Guarro, Josep
背景：泡囊霉属是毛霉目单型菌属。据报道，秀丽隐翅虫物种在免疫功能低下和免疫功能正常的人中引起严重感染。在Alvarez et al. (3)[J Clin Microbiol 2009;47：1650-6]，我们证明了A.材料和方法：我们进行了多相研究的基础上的组蛋白3基因的序列分析，rDNA基因的内部转录间隔区，和28 S rRNA基因的D1和D2域，以及通过评估的一些相关的形态和生理特征的一组临床和环境菌株的A。结果和结论：我们证明了A.线虫是一个复杂的物种。我们建议作为新的物种Apophysmallossiformis，其特征在于骨形的孢子囊孢子，Apophysomycesalziformis，具有椭圆形的孢子囊孢子，和Apophysomycesvariabilis，具有可变形状的孢子囊孢子。这些物种不能同化七叶皂苷，而A.秀丽隐杆线虫能够吸收这种糖苷。两性霉素B和泊沙康唑是体外抗真菌活性最高的药物。(C)2009年《伊比利亚美洲微生物学杂志》。出版社：Elsevier Espana，S.L. All rights reserved.
Background: Apophysomyces is a monotypic genus belonging to the order Mucorales. The species Apophysomyces elegans has been reported to cause severe infections in immunocompromised and immunocompetent people. In a previous study of Alvarez et al.(3) [J Clin Microbiol 2009;47:1650-6], we demonstrated a high variability among the 5.8S rRNA gene sequences of clinical strains of A. elegans.Material and methods: We performed a polyphasic study based on the analysis of the sequences of the histone 3 gene, the internal transcribed spacer region of the rDNA gene, and domains D1 and D2 of the 28S rRNA gene, as well as by evaluation of some relevant morphological and physiological characteristics of a set of clinical and environmental strains of A. elegans.Results and conclusions: We have demonstrated that A. elegans is a complex of species. We propose as new species Apophysmyces ossiformis, characterised by bone-shaped sporangiospores, Apophysomyces trapeziformis, with trapezoid-shaped sporangiospores, and Apophysomyces variabilis, with variable-shaped sporangiospores. These species failed to assimilate esculin, whereas A. elegans was able to assimilate that glycoside. Amphotericin B and posaconazole are the most active in vitro drugs against Apophysomyces. (C) 2009 Revista Iberoamericana de Micologia. Published by Elsevier Espana, S.L. All rights reserved.