Leu-M1--a marker for Reed-Sternberg cells in Hodgkin's disease. An immunoperoxidase study of paraffin-embedded tissues.
Leu-M1--a marker for Reed-Sternberg cells in Hodgkin's disease. An immunoperoxidase study of paraffin-embedded tissues.
复制标题
Leu-M1——霍奇金病中 Reed-Sternberg 细胞的标记。
作者：
G. Pinkus;P. Thomas;J. Said
Leu-M1是一种粒细胞相关分化抗原，其单克隆抗体是检测霍奇金病石蜡包埋组织中诊断性Reed-Sternberg（R-S）细胞及其变体的高效试剂。在73例何杰金氏病（41例结节硬化，25例混合细胞，4例淋巴细胞为主，3例淋巴细胞耗竭型）中，69例R-S细胞对Leu-M1呈强免疫反应性。4例淋巴细胞优势型霍奇金病（结节性）均为Leu-M1无反应。在大多数阳性病例（57/69，83%）中，大多数（60 - 90%）R-S细胞和变异体显示Leu-M1免疫反应性。特征性染色模式包括颗粒状和/或囊泡状胞质免疫反应性，通常具有突出的球状核旁反应产物，以及具有高度不规则胞质边界的膜染色。对B细胞（37例标本）、T细胞（20例标本）和真正组织细胞（3例标本）肿瘤和1例肥大细胞增多症的评价显示，仅在1例B细胞和4例T细胞恶性肿瘤中存在Leu-M1免疫反应性。然而，这些病例中的染色模式明显不同于R-S细胞。对非肿瘤性淋巴组织（共38例）的研究表明，淋巴细胞通常无反应性;组织细胞显示对Leu-M1的可变反应性。淋巴结窦状网络的偶尔组织细胞，特别是在弓形虫淋巴结炎中，表现出与R-S细胞相似的染色模式（膜/细胞质/核旁）。Leu-M1在评估霍奇金病及其与非霍奇金淋巴瘤和其他淋巴组织增生的区别中代表了一种潜在的有用的诊断判别。
Monoclonal antibody to Leu-M1, a granulocyte-related differentiation antigen, represents a highly effective reagent for detection of diagnostic Reed-Sternberg (R-S) cells and variants in paraffin-embedded tissues of Hodgkin's disease. In 69 of 73 cases of Hodgkin's disease (41 nodular sclerosis, 25 mixed cellularity, 4 lymphocyte predominance, and 3 lymphocyte depletion types), R-S cells were strongly immunoreactive for Leu-M1. Four cases of lymphocyte predominance Hodgkin's disease (nodular) were uniformly nonreactive for Leu-M1. In most of the positive cases (57/69, 83%), the majority (60-90%) of R-S cells and variants exhibited immunoreactivity for Leu-M1. A characteristic staining pattern included granular and/or vesicular cytoplasmic immunoreactivity, often with a prominent globular paranuclear reaction product, and membrane staining with highly irregular cytoplasmic borders. Evaluation of B-cell (37 specimens), T-cell (20 specimens), and true histiocytic (3 specimens) neoplasms and a case of mastocytosis revealed immunoreactivity for Leu-M1 only in 1 B-cell and 4 T-cell malignancies. The staining patterns in these cases, however, clearly differed from that observed for R-S cells. Studies of nonneoplastic lymphoid tissues (38 total) demonstrated that lymphoid cells were typically nonreactive; histiocytes revealed variable reactivity for Leu-M1. Occasional histiocytes of the sinusoidal network of lymph nodes, particularly in toxoplasmic lymphadenitis, exhibited a staining pattern (membranous/cytoplasmic/paranuclear) similar to that observed for R-S cells. Leu-M1 represents a potentially helpful diagnostic discriminant in the assessment of Hodgkin's disease and its distinction from non-Hodgkin's lymphomas and other lymphoid proliferations.
霍奇金病的组织化学。
DOI：
--
发表时间：
1982
期刊：
Cancer treatment reports
影响因子：
--
作者：
Beckstead,JH;Warnke,R;Bainton,DF
通讯作者：
Bainton,DF
霍奇金病的错误临床和病理诊断：西南肿瘤学小组研究。
DOI：
--
发表时间：
1982
期刊：
Cancer treatment reports
影响因子：
--
作者：
Miller,TP;Byrne,GE;Jones,SE
通讯作者：
Jones,SE
霍奇金病涉及的淋巴结和脾脏细胞成分的原位免疫学特征。
影响因子：
20.3
作者：
Poppema,S;Bhan,AK;Reinherz,EL;Posner,MR;Schlossman,SF
通讯作者：
Schlossman,SF