Effectiveness of 222-nm ultraviolet light on disinfecting SARS-CoV-2 surface contamination
Effectiveness of 222-nm ultraviolet light on disinfecting SARS-CoV-2 surface contamination
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DOI：
10.1016/j.ajic.2020.08.022
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发表时间：
2021-02-25
影响因子：
4.9
通讯作者：
Ohge, Hiroki
中科院分区：
文献类型：
--
作者：
Kitagawa, Hiroki;Nomura, Toshihito;Ohge, Hiroki
背景：引起2019年冠状病毒病(新冠肺炎)的严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)在全球范围内已成为对人类健康的严重威胁。对被SARS-CoV-2污染的表面进行有效消毒可能有助于防止其传播。目的：研究222 nm远紫外光(UVC)对SARS-CoV-2表面污染的体外消毒效果。方法：采用50%组织培养感染剂量(TCID50)，以222 nm波长照射10-300秒，检测SARS-CoV-2病毒滴度。结果：TCID50检测显示，1 mJ/cm2和3 mJ/cm2的222 nm UVC照射(0.1 mW/cm2，10 s和30 s)对SARS-CoV-2病毒的抑制率分别为88.5%和99.7%。相反，UVC照射5min后，SARS-CoV-2RNA的拷贝数也没有变化。结论：222 nm UVC照射在体外实验中显示了抗SARS-CoV-2污染的效果。还需要进一步评估222纳米紫外线照射在减少现实世界表面污染和SARS-CoV-2潜在传播方面的安全性和有效性。(C)2020年感染控制和流行病学专业人员协会，Elsevier Inc.出版。
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), has emerged as a serious threat to human health worldwide. Efficient disinfection of surfaces contaminated with SARS-CoV-2 may help prevent its spread. This study aimed to investigate the in vitro efficacy of 222-nm far-ultraviolet light (UVC) on the disinfection of SARS-CoV-2 surface contamination.Methods: We investigated the titer of SARS-CoV-2 after UV irradiation (0.1 mW/cm(2)) at 222 nm for 10-300 seconds using the 50% tissue culture infectious dose (TCID50). In addition, we used quantitative reverse transcription polymerase chain reaction to quantify SARS-CoV-2 RNA under the same conditions.Results: One and 3 mJ/cm(2) of 222-nm UVC irradiation (0.1 mW/cm(2) for 10 and 30 seconds) resulted in 88.5 and 99.7% reduction of viable SARS-CoV-2 based on the TCID50 assay, respectively. In contrast, the copy number of SARS-CoV-2 RNA did not change after UVC irradiation even after a 5-minute irradiation.Conclusions: This study shows the efficacy of 222-nm UVC irradiation against SARS-CoV-2 contamination in an in vitro experiment. Further evaluation of the safety and efficacy of 222-nm UVC irradiation in reducing the contamination of real-world surfaces and the potential transmission of SARS-CoV-2 is needed. (C) 2020 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc.