Ropivacaine inhibits neurite outgrowth in PC-12 cells
Ropivacaine inhibits neurite outgrowth in PC-12 cells
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DOI：
10.1213/01.ane.0000130650.47552.18
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发表时间：
2004-09-01
影响因子：
5.7
通讯作者：
Sumikawa, K
中科院分区：
文献类型：
--
作者：
Todoroki, S;Morooka, H;Sumikawa, K
周围神经损伤常常导致神经性疼痛，这可能涉及背根神经节中出芽的交感节后神经纤维。最近的研究表明罗哌卡因可有效阻止神经性疼痛。为了确定罗哌卡因是否影响交感神经萌芽，我们使用了嗜铬细胞瘤 (PC-12) 细胞，该细胞在接触神经生长因子 (NGF) 时分化为神经元。 PC-12 细胞在 50 ng/mL NGF 存在下预培养 4 天。神经突生长被量化为暴露于剂量范围为 10 至 200 微克/毫升的罗哌卡因 24、48 和 72 小时后的神经突延伸。罗哌卡姆以紧密依赖性的方式抑制神经突生长。罗哌卡因的抑制作用是完全可逆的，因为在洗掉罗哌卡因后，NGF 刺激的神经突生长恢复到对照水平。因此，罗哌卡因可能至少部分通过抑制交感神经萌芽来发挥其对神经性疼痛的治疗作用。
Peripheral nerve injury often leads to neuropathic pain, which might involve sympathetic postganglionic nerve fiber sprouting in the dorsal root ganglion. Recent studies suggest the effectiveness of ropivacaine in blocking neuropathic pain. To determine if ropivacaine affects sympathetic sprouting, we used pheochromocytoma (PC-12) cells, which differentiate into neurons on exposure to nerve growth factor (NGF). PC-12 cells were precultured in the presence of 50 ng/mL of NGF for 4 days. Neurite outgrowth was quantified as neurite extension after 24-, 48-, and 72-h exposure to ropivacaine at doses ranging from 10 to 200 mug/mL. Ropivacame inhibited the neurite outgrowth in a close-dependent manner. The inhibitory effect of ropivacaine was completely reversible because the NGF-stimulated neurite outgrowth was recovered to control level after washing out ropivacaine. Ropivacaine, therefore, may exert its therapeutic action on neuropathic pain, at least in part, by suppressing sympathetic sprouting.