A preliminary histological investigation of gall induction in an unconventional galling system
A preliminary histological investigation of gall induction in an unconventional galling system
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DOI：
10.1007/s11829-012-9193-4
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发表时间：
2012-09-01
影响因子：
1.6
通讯作者：
De Clerck-Floate, Rosemarie A.
中科院分区：
文献类型：
--
作者：
Barnewall, Emily C.;De Clerck-Floate, Rosemarie A.
在一个不寻常的情况下，涉及候选生物控制剂，组织学复杂的茎瘿象甲，Rhinusa pilosa（鞘翅目：刺蛾科）对黄色toadflax（柳穿鱼），迅速诱导产卵过程中，并达到全尺寸的幼虫孵化。为了研究瘿的诱导、产卵行为。pilosa描述。我们实验破坏产卵象鼻虫在产卵序列的三个关键点，并比较宿主植物组织的反应后中断，发生在正常的胆汁诱导，使用组织学方法。从头木质部的生产，在皮层中的细胞间隙，和增生和肥大的原形成层和髓薄壁组织周围的鸡蛋是一些组织和细胞水平的修改后，观察到只有3-5天正常产卵。在任何产卵干扰处理后都没有观察到正常的虫瘿发育，尽管一些细胞和组织反应类似于未中断产卵后发现的反应。在产卵道形成过程中，雌性取食诱导了伤口分生组织和愈伤组织的形成，但没有与虫瘿形成一致的其他变化。当雌性在产卵约20 s时被破坏时，观察到均匀致密的物质，这被怀疑是产卵液。有轻微的茎肿胀10天后，组织学上，平周细胞分裂，从头木质部，髓细胞与众多染色质体观察到在正常的虫瘿发育，从而表明产卵液在虫瘿诱导中发挥作用。
In an unusual case involving a candidate biological control agent, the histologically complex stem galls of the weevil, Rhinusa pilosa (Coleoptera: Curculionidae) on yellow toadflax (Linaria vulgaris), are rapidly induced during oviposition and reach full size by larval hatch. To investigate gall induction, the oviposition behavior of R. pilosa was described. We experimentally disrupted ovipositing weevils at three key points in the oviposition sequence and compared host-plant tissue responses post disruption, to what occurs during normal gall induction using histological methods. De novo xylem production, intercellular spaces in the cortex, and hyperplasia and hypertrophy of the procambium and pith parenchyma surrounding the egg were some of the tissue- and cellular-level modifications observed only 3-5 days after normal oviposition. Normal gall development was not observed after any of the oviposition disruption treatments, although some of the cellular and tissue responses resembled those found after undisrupted oviposition. Feeding by the female during oviposition canal formation induced wound meristem and callus tissue formation, but no other modifications consistent with gall formation. When females were disrupted about 20 s into oviposition, a homogenously dense substance was observed, which was suspected to be ovipositional fluid. There was minor stem swelling 10 days later and histologically, periclinal cell divisions, de novo xylem, and pith cells with numerous stained plastids were observed as in normal gall development, thus suggesting that ovipositional fluid plays a role in gall induction.