Prenatal stress reduces estradiol-induced prolactin release in male and female rats.
Prenatal stress reduces estradiol-induced prolactin release in male and female rats.
复制标题
产前应激会减少雄性和雌性大鼠中雌二醇诱导的催乳素释放。
DOI：
10.1016/0031-9384(87)90112-0
复制
发表时间：
1987
影响因子：
2.9
通讯作者：
Bridges,RS
中科院分区：
文献类型：
--
作者：
Kinsley,CH;Bridges,RS
产前压力是性分化正常过程的一个强有力的破坏者，对男性和女性都有影响。在本研究中，我们希望在产前应激(P-S)的男性和女性中检验性二态内分泌反应--雌二醇(E_2)诱导的催乳素(PRL)释放。SD雌性大鼠定时交配(+精子=第1天)。从妊娠15-22天开始，一组雌性大鼠接受每天三次的热和束缚应激(0830、1230和1630小时)，包括将大鼠放入一个有机玻璃束缚管中，管子上放置两个100W的泛光灯。对照雌鼠在整个怀孕期间保持不受干扰。在分娩时，所有的后代都被交叉养育到未经处理的新近分娩的母马，并在25天大时断奶。P-S组和对照组的男性和女性被摘除性腺，对于男性，记录成对的睾丸重量和体重。四天后，动物被植入含有E2的硅胶胶囊，并安装了心房内插管。第二天，分别在上午9点、13点、1500点和17点采集血液样本，共5天。从第2天的1700小时样本开始，除第3天的1500小时样本外，P-S雄性在整个第4天的血浆PRL值都显著低于对照组，在第5天的1500小时样本中也是如此。此外，P-S雄性每天下午的PRL值都没有显著增加，而对照组雄性在第2天和第4天有显著升高。P-S和对照组雄性在体重上没有差异，尽管P-S雄性的配对睾丸重量显著增加。产前应激的女性通常比对照组女性对E2对PRL释放的刺激效应反应较差，特别是表现出下午PRL激增的能力。在采样期的第1天和第2天，P-S雌虫和对照雌虫在任何时间点上没有差异。P-S雌性在第3-5天的两个下午晚些时候(1500-1700小时)的PRL水平显著降低，而在第5天的1300小时的样本中PRL水平显著升高。这些数据表明，产前应激影响雄性和雌性对雌雄动物分泌PRL的能力，并可能表明对中枢神经系统内分泌功能的影响，以补充先前报道的P-S动物的行为缺陷。
Prenatal stress is a potent disruptor of the normal course of sexual differentiation, affecting both males and females. In the present study, we wished to examine a sexually dimorphic endocrine response, estradiol (E2)-induced prolactin (Prl) release, in prenatally-stressed (P-S) males and females. Sprague-Dawley female rats were timed-mated ( + sperm=Day 1). From gestation days 15–22 one group of females was subjected to a thrice-daily regimen of heat and restraint stress (0830, 1230, and 1630 hr) consisting of placing the rats into a Plexiglas restraint tube over which were poised two 100 W floodlights. Control females remained undisturbed throughout pregnancy. At parturition all offspring were cross-fostered to untreated, recently-parturient dams and weaned at 25 days of age. Separate groups of P-S and Control males and females were gonadectomized and, for males, paired testes weights and body weights were recorded. Four days later the animals were implanted with Silastic capsules containing E2, and fitted with intra-atrial cannulae. The following day, blood samples were taken at 0900, 1300, 1500, and 1700 hr for a total of five days. Beginning with the 1700 hr sample on Day 2, and with the exception of the 1500 hr sample on Day 3, P-S males had significantly lower plasma Prl values than Control males through Day 4, and at the 1500 hr sample on Day 5. Moreover, at no point did P-S males exhibit a significant daily afternoon increase in Prl values, whereas Control males did so on Days 2 and 4. There was no difference between P-S and Control males in body weight, though P-S males had significantly heavier paired testes weights. Prenatally-stressed females were generally less responsive than Control females to the stimulatory effects of E2 on Prl release, in particular, the capacity to exhibit an afternoon Prl surge. The P-S and Control females did not differ at any time point on Days 1 and 2 of the sampling period. Nor were they different between 0900 and 1300 hr on Days 3 and 4, and 0900 hr on Day 5. P-S females exhibited significantly lower levels of Prl on the two late afternoon samples (1500–1700 hr) on Days 3–5, and had significantly higher levels at the 1300 hr sample on Day 5. These data demonstrate that prenatal stress affects the capacity of both males and females to secrete Prl in response to E2 and may indicate effects on central nervous system endocrinological functions complementary to behavioral deficits previously reported for P-S animals.