Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp cucumerinum
Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp cucumerinum
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DOI：
10.1002/jobm.201500666
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发表时间：
2016-08-01
影响因子：
3.1
通讯作者：
Yu, Xiaoping
中科院分区：
文献类型：
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作者：
Lu, Dandan;Ma, Zheng;Yu, Xiaoping
放线菌作为植物病原真菌的生物防治剂和植物生长促进剂受到了广泛的关注。本研究共分离到320株放线菌，来自中国不同的生境。其中77个菌株对尖孢镰刀菌具有拮抗活性。sp. cucumerinum，通常引起黄瓜枯萎病。其中，放线菌M527不仅表现出广谱的抗真菌活性，而且对F. oxysporum F.胡瓜黑腐病菌（sp.cucumerinum）盆栽试验结果表明，M527菌株对黄瓜新梢生长有一定的促进作用，对黄瓜枯萎病有一定的防治作用。oxysporum F.胡瓜黑腐病菌（sp.cucumerinum）M527发酵液灌根对黄瓜苗期枯萎病的防治效果达72.1%。根据16S rDNA序列分析，将分离物M527鉴定为龟裂链霉菌（Streptomyces rimosus）。
Actinomycetes have received considerable attention as biocontrol agents against fungal plant pathogens and as plant growth promoters. In this study, a total of 320 actinomycetes were isolated from various habitats in China. Among which, 77 strains have been identified as antagonistic activities against Fusarium oxysporum f. sp. cucumerinum which usually caused fusarium wilt of cucumber. Of these, isolate actinomycete M527 not only displayed broad-spectrum antifungal activity but also showed the strongest antagonistic activity against the spore germination of F. oxysporum f. sp. cucumerinum. In pot experiments, the results indicated that isolate M527 could promote the shoot growth and prevent the development of the disease on cucumber caused by F. oxysporum f. sp. cucumerinum. The control efficacy against seedling fusariumwilt of cucumber after M527 fermentation broth root-irrigation was up to 72.1% as compared to control. Based on 16S rDNA sequence analysis, the isolate M527 was identified as Streptomyces rimosus.