The design of polymer microcarrier surfaces for enhanced cell growth
The design of polymer microcarrier surfaces for enhanced cell growth
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DOI：
10.1016/s0142-9612(03)00319-3
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发表时间：
2003-10-01
期刊：
影响因子：
14
通讯作者：
Kunitake, M
中科院分区：
文献类型：
--
作者：
Kato, D;Takeuchi, M;Kunitake, M
制备了多种基于聚氨基酸的中性和阳离子聚合物，并研究了其作为细胞附着和生长的微载体。在包括烷基化聚（γ-甲基L-谷氨酸盐）（PG）颗粒（其中疏水性作为烷基长度的函数而变化）和羟基末端PG颗粒的中性聚合物颗粒中，具有最长烷基链的PG颗粒（PG-C12）表现出最高的细胞附着率和最高的细胞生长率。此外，羟基基团（PG-OH）的引入导致细胞生长的恶化。具有伯氨基的阳离子颗粒上的细胞生长强烈地依赖于阴离子交换容量（AEC）。胺化PG微载体的AEC较高，抑制细胞生长。相比之下，交联聚（ε-赖氨酸）（PL）微载体的AEC较高，有利于细胞生长。阳离子颗粒上的细胞生长清楚地显示出与微载体的pK（a，app）的良好相关性，但与它们的AEC无关。具有低和高pK（a）值的颗粒在表面附近分别具有有毒的酸性和碱性pH微环境。这些微环境具有细胞毒性作用。另一方面，没有观察到附着速率常数和高细胞生长之间的相关性。其中pK（a）被控制在中性pH的胺化颗粒和PG-C12产生比市售微载体明显更高的细胞生长。(C)2003爱思唯尔科技有限公司版权所有。
A variety of neutral and cationic polymers based on polyamino acids were prepared and investigated as microcarriers for cell attachment and growth. Among neutral polymer particles including the alkylated poly( gamma-methyl L-glutamate) (PG) particles, in which the hydrophobicity changes as a function of the length of the alkyl groups, and hydroxy terminal PG particles, the PG particle with the longest alkyl chain (PG-C12) demonstrated the highest cell attachment rate and highest rate of cell growth. Moreover, the introduction of hydroxyl groups (PG-OH) led to a deterioration of cell growth. Cell growth on cationic particles having primary amino groups was drastically dependent upon the anion exchange capacity (AEC). A higher AEC for aminated PG microcarriers inhibited cell growth. In contrast, a higher AEC for cross-linked poly(epsilon-lysine) (PL) microcarriers facilitated cell growth. Cell growth on cationic particles clearly showed a good correlation with the pK(a,app) of the microcarriers, but not with their AEC. The particles with low and high pK(a) values possessed toxically acidic and basic pH microenvironments near the surface, respectively. These microenvironments had cytotoxic effects. On the other hand, no correlation between attachment rate constants and high cell growth was observed. The aminated particles, in which pK(a) were controlled at neutral pH, and PG-C12 produced obviously higher cell growth than did a commercially available microcarrier. (C) 2003 Elsevier Science Ltd. All rights reserved.