Mucosal, Cellular, and Humoral Immune Responses Induced by Different Live Infectious Bronchitis Virus Vaccination Regimes and Protection Conferred against Infectious Bronchitis Virus Q1 Strain
Mucosal, Cellular, and Humoral Immune Responses Induced by Different Live Infectious Bronchitis Virus Vaccination Regimes and Protection Conferred against Infectious Bronchitis Virus Q1 Strain
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DOI：
10.1128/cvi.00368-15
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发表时间：
2015-09-01
影响因子：
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通讯作者：
Ganapathy, Kannan
中科院分区：
文献类型：
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作者：
Chhabra, Rajesh;Forrester, Anne;Ganapathy, Kannan
本研究的目的是评估两种不同的传染性支气管炎病毒（IBV）疫苗接种方案诱导的粘膜，细胞和体液免疫应答及其对变异IBV Q1攻击的有效性。一日龄的肉鸡用活H120单独（组I）或与CR88组合（组II）接种。两组在14日龄（doa）时再次接种CR88。另一组作为对照组（III组）。在接种组中，在4 doa时观察到泪液伊加水平显著增加，然后在14 doa时达到峰值。从14天开始，Ⅱ组伊加水平明显高于Ⅰ组。使用免疫组织化学检查气管中CD4（+）和CD8（+）细胞数量的变化，发现在两个接种组中，与CD4（+）细胞相比，CD8（+）细胞的总体模式占主导地位。在21和28 doa时，II组的CD8（+）细胞显著高于I组。在28 doa用强毒Q1菌株对所有组进行眼鼻攻击，并评估其保护作用。两个接种组对Q1提供了极好的纤毛保护，尽管组II的组织病理学病变评分和气管和肾脏中的病毒RNA载量显示出比组I更高的保护水平。这些结果表明，从1日龄雏鸡的H120和CR88的组合疫苗接种，然后在14 doa接种CR88，实现了更大的保护。
The objectives of the present study were to assess the mucosal, cellular, and humoral immune responses induced by two different infectious bronchitis virus (IBV) vaccination regimes and their efficacy against challenge by a variant IBV Q1. One-day-old broiler chicks were vaccinated with live H120 alone (group I) or in combination with CR88 (group II). The two groups were again vaccinated with CR88 at 14 days of age (doa). One group was kept as the control (group III). A significant increase in lachrymal IgA levels was observed at 4 doa and then peaked at 14 doa in the vaccinated groups. The IgA levels in group II were significantly higher than those in group I from 14 doa. Using immunohistochemistry to examine changes in the number of CD4(+) and CD8(+) cells in the trachea, it was found that overall patterns of CD8(+) cells were dominant compared to those of CD4(+) cells in the two vaccinated groups. CD8(+) cells were significantly higher in group II than those in group I at 21 and 28 doa. All groups were challenged oculonasally with a virulent Q1 strain at 28 doa, and their protection was assessed. The two vaccinated groups gave excellent ciliary protection against Q1, although group II's histopathology lesion scores and viral RNA loads in the trachea and kidney showed greater levels of protection than those in group I. These results suggest that greater protection is achieved from the combined vaccination of H120 and CR88 of 1-day-old chicks, followed by CR88 at 14 doa.