Prostaglandin synthesis by isolated rat glomeruli: effect of angiotensin II.
Prostaglandin synthesis by isolated rat glomeruli: effect of angiotensin II.
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离体大鼠肾小球的前列腺素合成：血管紧张素 II 的作用。
DOI：
10.1152/ajprenal.1980.239.5.f486
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Folkert,VW
中科院分区：
文献类型：
--
作者：
Schlondorff,D;Roczniak,S;Satriano,JA;Folkert,VW
前列腺素在肾血流和肾小球滤过的调节中起作用。在[14 C]花生四烯酸存在下，离体肾小球产生的前列腺素的模式为PGF 2 α> PGE 2> PGD 2 = TXB 2 = 6-keto-PGF 1 α（前列环素的代谢产物）。用[14 C]花生四烯酸盐预标记的肾小球显示出额外的标记前列腺素，其与6，15-二酮-13，13-二氢-PGF 1 α共色谱，可能代表前列环素的分解产物。因此，根据其分解产物判断，前列环素是产生的第二丰富的前列腺素。这些结果通过PGF 2 α、PGE 2和6-keto-PGF 1 α的特异性放射免疫测定得到证实。分离的肾小球产生1，740 pg x 10 min-1 x mg蛋白-1的PGF 2 α、798 pg x 10 min-1 x mg蛋白-1的6-keto-PGF 1 α和266 od PGE 2。在预先标记的肾小球血管紧张素II导致一个小的，但显着增加14 C-标记的arglandins。6-keto-PGF 1 α的放射免疫分析表明，血管紧张素刺激对前列环素具有特异性。血管紧张素II也影响[14 C]花生四烯酸的肾小球处理。它减少了细胞外[14 C]花生四烯酸的摄取，并增加了细胞内[14 C]花生四烯酸掺入肾小球磷脂。基于这些结果，我们提出，在肾小球血管紧张素增加前列腺素的合成和刺激磷脂的脱酰化和再酰化。
Prostaglandins play a role in the regulation of renal blood flow and glomerular filtration. In the presence of [14C]arachidonate the pattern of prostaglandins produced by isolated glomeruli was PGF2 alpha > PGE2 > PGD2 = TXB2 = 6-keto-PGF1 alpha (a metabolite of prostacyclin). Glomeruli prelabeled with [14C]arachidonate showed an additional labeled prostaglandin that co-chromatographs with 6,15-diketo-13,13-dihydro-PGF1 alpha and may represent breakdown product of prostacyclin. Thus, prostacyclin, judged by its breakdown products, was the second most abundant prostaglandin produced. These results were confirmed by specific radioimmunoassays for PGF2 alpha, PGE2, and 6-keto-PGF1 alpha. Isolated glomeruli produced 1,740 pg x 10 min-1 x mg protein-1 of PGF2 alpha, 798 of 6-keto-PGF1 alpha, and 266 od PGE2. In prelabeled glomeruli angiotensin II causes a small but significant increase in 14C-labeled prostaglandins. Radioimmunoassay for 6-keto-PGF1 alpha showed that the angiotensin stimulation was specific for prostacyclin. Angiotensin II also affected the glomerular handling of [14C]arachidonate. It decreased the uptake of extracellular [14C]arachidonate and increased the incorporation of intracellular [14C]arachidonate into glomerular phospholipids. Based on these results, we propose that in the glomerulus angiotensin increases prostaglandin synthesis and stimulates deacylation and reacylation of phospholipids.