Visualization of G-quadruplexes by using a BODIPY-labeled macrocyclic heptaoxazole.
Visualization of G-quadruplexes by using a BODIPY-labeled macrocyclic heptaoxazole.
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DOI：
10.1039/c002117b
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发表时间：
2010-06
影响因子：
3.2
通讯作者：
Masayuki Tera;Keisuke Iida;K. Ikebukuro;H. Seimiya;K. Shin‐ya;K. Nagasawa
中科院分区：
文献类型：
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作者：
Masayuki Tera;Keisuke Iida;K. Ikebukuro;H. Seimiya;K. Shin‐ya;K. Nagasawa
开发了BODIPY标记的大环七恶唑L1 BOD-7 OTD作为G-四链体的荧光配体。研究结果表明，L1 BOD-7 OTD都选择性地诱导某些G-四链体形成寡核苷酸（GFO）形成分子内G-四链体。此外，标记的大环heptaozaxole强烈结合并稳定分子内G-四链体。此外，该物质可用于以绿色荧光的形式直接显现G-四链体。最后，利用L1 BOD-7 OTD证明了G-四链体在细胞中形成的可能性。
A BODIPY-labeled macrocyclic heptaoxazole, L1BOD-7OTD, was developed as a fluorescent ligand for G-quadruplexes. The results of the study show that L1BOD-7OTD both selectively induces the formation of intramolecular G-quadruplexes from some G-quadruplex forming oligonucleotides (GFOs). In addition, the labelled macrocyclic heptaozaxole strongly binds to and stabilizes intramolecular G-quadruplexes. Moreover, this substance can be used to directly visualize the G-quadruplexes in the form of green fluorescence. Finally, the possibility that G-quadruplexes form in the cells was demonstrated by using of L1BOD-7OTD.