Expression Analysis of mRNAs in Oral Squamous Cell Carcinoma Using Laser Microdissection (Laser Pressure Cell Transfer Type) and Semiquantitative RT-PCR
Expression Analysis of mRNAs in Oral Squamous Cell Carcinoma Using Laser Microdissection (Laser Pressure Cell Transfer Type) and Semiquantitative RT-PCR
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使用激光显微切割（激光压力细胞转移型）和半定量 RT-PCR 对口腔鳞状细胞癌中 mRNA 的表达进行分析
DOI：
10.11516/dentalmedres1981.21.75
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
T. Tachikawa
中科院分区：
文献类型：
--
作者：
T. Irié;Reiko Tsuchiya;G. Yamamoto;Y. Maeda;T. Tachikawa
激光显微切割是一种从组织切片中获取靶细胞的新方法，并可用于核酸和蛋白质的分析。然而，由于传统激光显微切割在解剖后必须将靶细胞粘附到热塑性薄膜上，因此除靶细胞外的细胞污染和mRNA损伤已成为问题。我们开发了一种不需要使用热塑性薄膜的激光压力细胞转移方法，并利用该方法和半定量RT-PCR对同一样本中口腔鳞状细胞癌和正常粘膜上皮中的各种转录产物进行了半定量分析。该分析的结果是，可以从每个样品中获取 mRNA。通过该分析积累的病例，表明可以成为对癌症恶性程度进行客观判断和预后评估的基础数据。
Laser microdissection is a new method for the procurement of targeted cells from tissue section and has enabled the analysis of nucleic acid and protein. However, the contamination of cells, other than target cells and damage of mRNA, has become a problem, since it must adhere the target cells to thermoplastic film after dissection in the conventional laser microdissection. We developed a laser pressure cell transfer method that did not need to use thermoplastic film, and the semiquantitative analysis of the various transcriptional product in oral squamous cell carcinoma and normal mucosal epithelium in the identical sample was carried out by using this method and semiquantitative RT-PCR. As a result of this analysis, the procurement of mRNA from each sample was possible. By accumulating of cases using this analysis, it was indicated to become basic data for the establishment of objective judgment and prognostic evaluation of the cancer's malignancy.