Nanowire biosensors for label-free, real-time, ultrasensitive protein detection.
Nanowire biosensors for label-free, real-time, ultrasensitive protein detection.
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用于无标记、实时、超灵敏蛋白质检测的纳米线生物传感器。
DOI：
10.1007/978-1-61779-319-6_18
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Lieber,CharlesM
中科院分区：
文献类型：
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作者：
Zheng,Gengfeng;Lieber,CharlesM
蛋白质的灵敏和定量分析是疾病诊断、药物筛选和蛋白质组学研究的核心。在利用新的纳米材料进行生物分子分析的最新研究进展中，硅纳米线（SiNWs）被配置为场效应晶体管（FET），已成为最有前途和最强大的平台之一，用于蛋白质以及许多其他生物物种的无标记，实时和高灵敏度的电检测。在这里，我们描述了一个详细的协议，实现硅纳米线生物传感器的蛋白质检测，包括硅纳米线合成，FET器件制造，表面受体功能化，和电感测测量。此外，在同一传感器阵列中结合p型和n型SiNW两者提供了用于感测信号验证的内部控制的独特手段。
Sensitive and quantitative analysis of proteins is central to disease diagnosis, drug screening, and proteomic studies. Among recent research advances exploiting new nanomaterials for biomolecule analysis, silicon nanowires (SiNWs), which are configured as field-effect transistors (FETs), have emerged as one of the most promising and powerful platforms for label-free, real-time, and highly sensitive electrical detection of proteins as well as many other biological species. Here, we describe a detailed protocol for realizing SiNW biosensors for protein detection that includes SiNW synthesis, FET device fabrication, surface receptor functionalization, and electrical sensing measurements. Moreover, incorporating both p-type and n-type SiNWs in the same sensor array provides a unique means of internal control for sensing signal verification.