An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
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DOI：
10.1186/1471-2407-10-336
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发表时间：
2010-06-28
期刊：
影响因子：
3.8
通讯作者：
Espinosa, Enrique
中科院分区：
文献类型：
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作者：
Sanchez-Navarro, Iker;Gamez-Pozo, Angelo;Espinosa, Enrique
背景：基因表达分析可以提高早期乳腺癌患者的预后准确性。我们的目标是证明可以开发一个简单的分子特征来预测远处复发。方法：我们包括153例I-I-II期激素受体阳性乳腺癌患者。从孔蛋白固定的石蜡嵌入样品中分离RNA，并用基因表达测定进行了83个基因的QRT-PCR扩增。我们分析的基因是70基因签名，复发评分和两基因指数中的基因。使用COX回归模型分析了基因表达，临床变量和遥远转移生存的关联。分析：定义了8基因预后评分。 5年的远处转移生存率为97％的患者被预后评分定义为低风险的患者，而定义为高风险的患者为60％。 8基因分数仍然是多变量分析的重要因素，其性能与两个经过验证的基因概况相似：70基因的签名和复发分数。结论：本研究确定了一个简单的基因表达评分，可以在乳腺癌中补充组织病理学的预后因素，并可以在嵌入的石蜡嵌入的样品中确定，该研究鉴定了一个简单的基因表达评分。
Background: Gene expression profiling may improve prognostic accuracy in patients with early breast cancer. Our objective was to demonstrate that it is possible to develop a simple molecular signature to predict distant relapse.Methods: We included 153 patients with stage I-II hormonal receptor-positive breast cancer. RNA was isolated from formalin-fixed paraffin- embedded samples and qRT-PCR amplification of 83 genes was performed with gene expression assays. The genes we analyzed were those included in the 70-Gene Signature, the Recurrence Score and the Two-Gene Index. The association among gene expression, clinical variables and distant metastasis-free survival was analyzed using Cox regression models.Results: An 8-gene prognostic score was defined. Distant metastasis-free survival at 5 years was 97% for patients defined as low-risk by the prognostic score versus 60% for patients defined as high-risk. The 8-gene score remained a significant factor in multivariate analysis and its performance was similar to that of two validated gene profiles: the 70-Gene Signature and the Recurrence Score. The validity of the signature was verified in independent cohorts obtained from the GEO database.Conclusions: This study identifies a simple gene expression score that complements histopathological prognostic factors in breast cancer, and can be determined in paraffin- embedded samples.