Adrenal steroid receptor binding in spleen and thymus after stress or dexamethasone.
Adrenal steroid receptor binding in spleen and thymus after stress or dexamethasone.
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应激或地塞米松后，肾上腺类固醇受体与脾脏和胸腺结合。
DOI：
10.1152/ajpendo.1990.259.3.e405
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
McEwen,BS
中科院分区：
文献类型：
--
作者：
Miller,AH;Spencer,RL;Stein,M;McEwen,BS
I型和II型肾上腺皮质类固醇受体结合测定肾上腺切除（ADX）大鼠和完整大鼠的脾脏和胸腺皮质酮的基础水平后，1小时的束缚应激或外源性给药后的地塞米松（DEX）。同时在海马和垂体中进行受体测定。免疫组织和脑垂体中的受体结合措施对不同水平的内源性激素的反应低于海马中的结合措施。与ADX大鼠相比，I型结合在脾脏和垂体的完整大鼠在基础水平的皮质酮是不变的，而I型结合在海马中显着下降。此外，尽管峰值水平的皮质酮，II型结合在脾脏，胸腺和垂体的应激大鼠也没有变化，而II型结合在海马的应激动物显着降低。相比之下，DEX，一种众所周知的免疫抑制剂，减少II型结合在免疫组织中比在海马。由于在体外测定的受体结合减少可能反映了体内受体活化，这些结果表明，内源性和外源性糖皮质激素在免疫，垂体和海马组织中肾上腺类固醇受体亚型的活化程度可能有相当大的异质性。
Type I and II adrenal steroid receptor binding was measured in spleen and thymus of adrenalectomized (ADX) rats and intact rats at basal levels of corticosterone after 1 h of restraint stress or after exogenous administration of dexamethasone (DEX). Concurrent receptor determinations were made in the hippocampus and pituitary. Receptor binding measures in immune tissues and pituitary were less responsive to varying levels of endogenous hormones than binding measures in hippocampus. Compared with ADX rats, type I binding in spleen and pituitary of intact rats at basal levels of corticosterone was unchanged, whereas type I binding in the hippocampus was significantly decreased. Furthermore, despite peak levels of corticosterone, type II binding in spleen, thymus, and pituitary of stressed rats was also unchanged, whereas type II binding in the hippocampus of stressed animals was significantly lower. In contrast, DEX, a well-known immunosuppressant, reduced type II binding in immune tissues more than in the hippocampus. Because a decrease in receptor binding measured in vitro may reflect receptor activation in vivo, these results suggest that there may be considerable heterogeneity in the degree of activation of adrenal steroid receptor subtypes in immune, pituitary, and hippocampal tissue by endogenous and exogenous glucocorticoids.