Myocardial perfusion and intracapillary blood volume in rats at rest and with coronary dilatation: MR imaging in vivo with use of a spin-labeling technique
Myocardial perfusion and intracapillary blood volume in rats at rest and with coronary dilatation: MR imaging in vivo with use of a spin-labeling technique
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DOI：
10.1148/radiology.215.1.r00ap07189
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发表时间：
2000-04-01
期刊：
影响因子：
19.7
通讯作者：
Bauer, WR
中科院分区：
文献类型：
--
作者：
Waller, C;Kahler, E;Bauer, WR
目的：验证一种不是第一次通过的磁共振成像技术，该技术可以显示完整大鼠的血流灌注和局部血容量(RBV)。材料和方法：血流灌注的测量是基于水质子磁自旋标记后的灌注敏感的T1弛豫。REV是根据血管内造影剂注射前后T1的稳态测量来确定的。采用彩色微球技术作为血流灌注测量的参照物。结果：在MR成像条件下，静息状态下的灌注量为3.5mL/g/min+/-0.1(扫描电子显微镜)，REV为11.6%+/-0.6(扫描电子显微镜)。磷酸腺苷显著增加灌注量至4.5毫升/克/分+/-0.3(扫描电子显微镜)，并将平均动脉压从120毫米汞柱降至65毫米汞柱，这意味着冠脉阻力降低到基线的40%。REV持续上升至23.8%+/-0.6(扫描电子显微镜)。结论：本研究结果表明，MR成像可以无创地对完整动物进行血流灌注和REV的定量标测。该方法可以确定血管扩张和灌流储备，反映了特定刺激下冠脉微循环的活体调节。
PURPOSE: To validate a magnetic resonance (MR) imaging technique that is not first pass and that reveals perfusion and regional blood volume (RBV) in the intact rat.MATERIALS AND METHODS: Measurement of perfusion was based on the perfusion-sensitive T1 relaxation after magnetic spin labeling of water protons. REV was determined from steady-state measurements of T1 before and after administration of an intravascular contrast agent. The colored microsphere technique was used as a reference method for perfusion measurement. REV and perfusion maps were obtained with the rats at rest and during administration of 3 mg of adenosine phosphate per kilogram of body weight per min ute.RESULTS: At MR imaging, perfusion during resting conditions was 3.5 mL/g/min +/- 0.1 (SEM), and REV was 11.6% +/- 0.6 (SEM). Adenosine phosphate significantly increased perfusion to 4.5 mL/g/min +/- 0.3 (SEM) and decreased mean arterial pressure from 120 mm Hg to 65 mm Hg, which implies a reduction of coronary resistance to 40% of baseline. REV increased consistently to 23.8% +/- 0.6 (SEM).CONCLUSION: The study results show that quantitative mapping of perfusion and REV may be performed noninvasively by means of MR imaging in the intact animal. The presented method allows determination of vasodilative and perfusion reserve, which reflects the in vivo regulation of coronary microcirculation for a given stimulus.