Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion.
Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion.
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DOI：
10.1038/s41598-017-00578-x
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发表时间：
2017-03-28
影响因子：
4.6
通讯作者：
Kamoun S
中科院分区：
文献类型：
--
作者：
Nekrasov V;Wang C;Win J;Lanz C;Weigel D;Kamoun S
基因组编辑已经成为一种有可能彻底改变植物育种的技术。在这项研究中，我们报告了在不到10个月的时间内，使用CRISPR/Cas9技术产生Tomelo，一种抗白粉病真菌病原体的非转基因番茄品种。我们使用全基因组测序来显示Tomelo不携带任何外源DNA序列，而只携带与自然发生的突变难以区分的缺失。我们还提供了CRISPR/Cas9是一种高度精确的工具的证据，因为我们没有在Tomelo中检测到脱靶突变。使用我们的管道，可以在不到一年的时间内以相对最小的努力和投资将突变引入精英或当地适应的番茄品种。
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.