Preparation of pH-stimuli-responsive PEG-TGA/TGH-capped CdTe QDs and their application in cell labeling
Preparation of pH-stimuli-responsive PEG-TGA/TGH-capped CdTe QDs and their application in cell labeling
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pH刺激响应的PEG-TGA/TGH封端的CdTe量子点的制备及其在细胞标记中的应用。
DOI：
10.1002/bio.2770
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发表时间：
2015-08-01
期刊：
影响因子：
2.9
通讯作者：
Tang, Daoquan
中科院分区：
文献类型：
--
作者：
Du, Yan;Yang, Dongzhi;Tang, Daoquan
以巯基乙酸（TGA）和巯基乙酰肼（TGH）为稳定剂，在水溶性体系中设计并合成了一种ph敏感的双功能纳米探针。TGA具有生物相容性，因为羧基很容易与生物大分子连接。同时，TGH上的肼与聚乙二醇（PEG）上的醛反应形成腙键。在特定的pH值下，腙键断裂，表现出pH刺激响应特性。作为光学成像探针，PEG-TGA/ tgh包封的CdTe量子点（QDs）具有高质量，荧光效率为25-30%，并且保持稳定至少5个月。该ph响应因子可用于肿瘤细胞酸性间质胞外环境下CdTe量子点的有效释放。这使得制备的ph刺激响应纳米探针能够显示荧光信号，用于癌细胞成像。
A pH-sensitive and double functional nanoprobe was designed and synthesized in a water-soluble system using thioglycolic acid (TGA) and mercapto-acetohydrazide (TGH) as the stabilizers. TGA is biocompatible because the carboxyl group is easily linked to biological macromolecules. At the same time, the hydrazide on TGH reacts with the aldehyde on poly(ethylene glycol) (PEG) and forms a hydrazone bond. The hydrazone bond ruptured at specific pH values and exhibited pH-stimuli-responsive characteristics. As an optical imaging probe, the PEG-TGA/TGH-capped CdTe quantum dots (QDs) had high quality, with a fluorescence efficiency of 25-30%, and remained stable for at least five months. This pH-responsive factor can be used for the effective release of CdTe QDs under the acidic interstitial extracellular environment of tumor cells. This allows the prepared pH-stimuli-responsive nanoprobes to show fluorescence signals for use in cancer cell imaging.