G1 shortening following unbalanced growth: a specific v. nonspecific effect.
G1 shortening following unbalanced growth: a specific v. nonspecific effect.
复制标题
生长不平衡后 G1 缩短：特定效应与非特定效应。
DOI：
10.1111/j.1365-2184.1992.tb01400.x
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发表时间：
1992
影响因子：
8.5
通讯作者：
RotiRoti,JL
中科院分区：
文献类型：
--
作者：
Goswami,PC;Higashikubo,R;Tolmach,LJ;RotiRoti,JL
The synthesis and abundance of proteins were examined in synchronous populations of HeLa cells under conditions in which the lengthening of S phase, by inhibiting DNA synthesis, resulted in shortening ofG1in the subsequent generation. Mitotically collected cells were resynchronized by incubation with 3 PM aphidicolin from 3 to 12 h after mitotic selection; they were blocked again at various times thereafter to induce unbalanced growth. Cells were labelled with [35S]‐methionine before and after release from the block to study the changes in protein synthesis. Triton X‐100 soluble and insoluble proteins were analysed by 7–15% gradient SDS‐PAGE, and radioactivity incorporation was quantified by liquid‐scintillation counting. The degree of G1shortening correlated with S phase position, increasing gradually from early S and reaching maximum when cells were blocked half‐way through S phase. Synthesis of proteins of 120, 66, and 51 kDa was stimulated, and synthesis of a new protein of 57kDa was observed, in cells in which DNA synthesis had been blocked in mid‐S. These proteins also showed increased accumulation. These results suggest that the shortening of GI, induced by the prior arrest of cell‐cycle progression, is associated with synthesis of specific proteins rather than the non‐specific accumulation of cellular proteins through unbalanced growth.
