The production mechanism and immunosuppression effect of pulmonary surfactant protein D via toll like receptor 4 signaling pathway in human corneal epithelial cells during Aspergillus fumigatus infection
The production mechanism and immunosuppression effect of pulmonary surfactant protein D via toll like receptor 4 signaling pathway in human corneal epithelial cells during Aspergillus fumigatus infection
复制标题
烟曲霉感染时人角膜上皮细胞中肺表面活性蛋白D通过Toll样受体4信号通路的产生机制及免疫抑制作用
DOI：
10.1016/j.intimp.2015.10.018
复制
发表时间：
2015-12-01
影响因子：
5.6
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
--
作者：
Wu, Xian;Zhao, Guiqiu;Zhang, Yan
Objective: To observe the production mechanism of surfactant protein D (SP-D) in human corneal epithelial cells (HCECs) when infected by Aspergillus fumigatus (A. fumigatus) hyphae, and explore whether SP-D can inhibit the cell activations through toll-like receptor 4 signaling pathway during fungal infection.Methods: mRNA and protein expressions of SP-D were evaluated in HCECs after stimulation by A. fumigatus, with or without pretreatment of TLR4 inhibitor (CLI-095) by real time PCR and Western blot. The expression levels of inflammatory cytokines IL-1 beta and IL-8 evaluated when pretreated with SP-D antibody or recombinant human SP-D in fungi-stimulated HCECs by real time PCR and ELISA, IL-1 beta and IL-8 expressions were also detected in A. fumigatus-stimulated HCECs that pretreated with CLI095 or MyD88 inhibitor (Pepinh-MYD) and recombinant human SP-D.Results: mRNA and protein levels of SP-D increased after stimulation of A. fumigatus for 16 hand 20 h respectively. The upregulation of SP-D could be inhibited by CLI-095. mRNA and protein expressions of IL-1 beta and IL-8 decreased significantly when pretreated HCECs with recombinant human SP-D for 4 h before A. fumigatus stimulation, while IL-113 and IL-8 increased when pretreated with SP-D antibody for 1 h. Pretreatment of CLI095 or Pepinh-MYD can increase the expressions of IL-1 beta and IL-8 mRNA and protein in HCECs induced by recombinant human SP-D and A. fumigatus.Conclusions: SP-D can be stimulated by TLR4 during A. fumigatus infection. Recombinant human SP-D can inhibit the expression of inflammatory cytokines through TLR4 signaling pathway. (C) 015 Elsevier B.V. All rights reserved.
