DNA of Epstein-Barr virus. III. Identification of restriction enzyme fragments that contain DNA sequences which differ among strains of Epstein-Barr virus
DNA of Epstein-Barr virus. III. Identification of restriction enzyme fragments that contain DNA sequences which differ among strains of Epstein-Barr virus
复制标题
Epstein-Barr 病毒的 DNA。
DOI：
10.1128/jvi.27.2.388-398.1978
复制
发表时间：
1978
影响因子：
5.4
通讯作者：
E. Kieff
中科院分区：
文献类型：
--
作者：
N. Raab;Randall Pritchett;E. Kieff
以前的动力学和吸收杂交实验已经证明，EB病毒B95-8株的DNA缺失了HR-1株DNA中约10%的DNA序列(R.F.Pritchett，S.D.Hayward和E.Kief，J.Virol)。15：556-569,1975；B.Sugder，W.C.Summers，和G.Klein，J.Virol。1976年18：765-775)。HR-1毒株不同于其他实验室毒株，包括B95-8和W91毒株，也不同于传染性单核细胞增多症患者的洗喉液中存在的病毒，因为它不能将淋巴细胞转化为能够在培养中长期生长的淋巴母细胞(P.Gerber，Lancet I：1001,1973；J.Meneze，W.Leibold，和G.Klein，Exp.牢房。Res.92：478-484,1975；G.Miller，D.Coope，J.Niederman，和J.Pagano，J.Virol。18：1071-1080,1976；G.Miller、J.Robinson、L.Heston和M.Lipman，Proc.娜塔莉。阿卡德。SCI。美国71：4006-4010,1974)。在本文报道的实验中，已经鉴定了EB病毒DNA的限制性内切酶片段，其包含的序列在HR-1、B95-8和W91株之间不同。HR-1、B95-8和W91株的DNA在复杂性上各有不同。以前在B95-8毒株中缺失的序列包含在HR-1毒株的EcoRI-C和-D和Hsu I-E和-N片段以及W91毒株的EcoRI-C和Hsu I-D和-E片段中。HR-1株缺失B95-8株EcoRI片段A、J至K、Hsu I片段B，以及W91株EcoRI-A、Hsu I-B片段。讨论了这些数据与B95-8株和W91株DNA限制性内切酶片段的连锁图的关系(E.Kief，N.Raab-Traub，D.Ged，W.King，A.T.Powell，R.Pritchett和T.Dambaugh，在F.Rapp和G.de-the，Oncogensis and HepesVirus III，in Press；D.Ged和E.Kief，提交发表)，以及这些数据的可能意义。
Previous kinetic and absorption hybridization experiments had demonstrated that the DNA of the B95-8 strain of Epstein-Barr virus was missing approximately 10% of the DNA sequences present in the DNA of the HR-1 strain (R.F. Pritchett, S.D. Hayward, and E. Kieff, J. Virol. 15:556-569, 1975; B. Sugder, W.C. Summers, and G. Klein, J. Virol. 18:765-775, 1976). The HR-1 strain differs from other laboratory strains, including the B95-8 and W91 strains, and from virus present in throat washings from patients with infectious mononucleosis in its inability to transform lymphocytes into lymphoblasts capable of long-term growth in culture (P. Gerber, Lancet i:1001, 1973; J. Menezes, W. Leibold, and G. Klein, Exp. Cell. Res. 92:478-484, 1975; G. Miller, D. Coope, J. Niederman, and J. Pagano, J. Virol. 18:1071-1080, 1976; G. Miller, J. Robinson, L. Heston, and M. Lipman, Proc. Natl. Acad. Sci. U.S.A. 71:4006-4010, 1974). In the experiments reported here, the restriction enzyme fragments of Epstein-Barr virus DNA which contain sequences which differ among the HR-1, B95-8, and W91 strains have been identified. The DNA of the HR-1, B95-8, and W91 strains each differed in complexity. The sequences previously shown to be missing in the B95-8 strain were contained in the EcoRI-C and -D and Hsu I-E and -N fragments of the HR-1 strain and in the EcoRI-C and Hsu I-D and -E fragments of the W91 strain. The HR-1 strain was missing DNA contained in EcoRI fragments A and J through K and Hsu I fragment B of the B95-8 strain and in the EcoRI-A and Hsu I-B fragments of the W91 strain. The relationship of these data to the linkage map of restriction enzyme fragments of the DNA of the B95-8 and W91 strains (E. Kieff, N. Raab-Traub, D. Given, W. King, A.T. Powell, R. Pritchett, and T. Dambaugh, In F. Rapp and G. de-The, ed., Oncogenesis and Herpesviruses III, in press; D. Given and E. Kieff, submitted for publication) and the possible significance of the data are discussed.