Identification of Pseudomonas syringae pv. syringae Causing Pear Blossom and Bud Blast in China
Identification of Pseudomonas syringae pv. syringae Causing Pear Blossom and Bud Blast in China
复制标题
丁香假单胞菌 pv. 的鉴定
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Qiang Jun
中科院分区：
文献类型：
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作者：
Qiang Jun
【目的】明确我国梨花和芽瘟病的病原微生物。 【方法】通过细菌学试验、电镜观察、科赫假设试验、Biolog、脂肪酸甲酯（FAMEs）和PCR试验等方法对病原菌进行鉴定，并与标准菌株进行比较。 【结果】16份病样品中20株致病菌中，6株代表性菌株与3株丁香假单胞菌标准菌株表现出相似的特征。来自比利时的丁香。它们被鉴定为丁香假单胞菌 pv。丁香菌的 Biolog 相似度为 0.57-0.86，FAME 相似度为 0.58-0.81。该细菌是从有症状的植物和花朵中重新分离出来的。鉴定为丁香假单胞菌 pv。结合上述结果，通过PCR引物和序列测试，确认丁香菌属。 【结论】已证实我国梨花芽瘟病可能是由丁香假单胞菌引起的。丁香科。
【Objective】This study was made to determine the causal organism of pear blossom and bud blast in China. 【Method】 The causal organism was identified by bacteriological test, electro- microscopic observation, Koch’s postulation test, Biolog, fatty acid methyl esters (FAMEs) and PCR test and compared with the standard strains【.Result】Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to 3 standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequences test and combined with the above results. 【Conclusion】 It has been confirmed that the pear blossom and bud blast in China may caused by P. syringae pv. syringae.