Multicolor fluorescence in situ hybridization for the simultaneous detection of probe sets for chromosome 13, 18, 21, X and Y in uncultured amniotic fluid cells
Multicolor fluorescence in situ hybridization for the simultaneous detection of probe sets for chromosome 13, 18, 21, X and Y in uncultured amniotic fluid cells
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DOI：
10.1093/hmg/1.5.307
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发表时间：
1992-01-01
影响因子：
3.5
通讯作者：
Ward, David C.
中科院分区：
文献类型：
--
作者：
Ried, Thomas;Landes, Greg;Ward, David C.
新生儿中最常见的非整倍体包括常染色体13、18和21以及两个性染色体。荧光原位杂交很容易在细胞周期的所有阶段检测到数值染色体畸变。利用基于组合探针标记和数字成像显微镜的多色荧光原位杂交方法，我们展示了针对染色体13、18、21、X和y的探针集的同时可视化。这种方法使人们能够利用来自各种细胞类型（包括淋巴细胞和羊膜细胞）的中期染色体和间期细胞核在一次杂交实验中评估多条染色体的畸变。
The most frequent aneuploidies in newborns involve the autosomes 13, 18 and 21 as well as both sex chromosomes. Fluorescence in situ hybridization readily allows the detection of numerical chromosomal aberrations throughout all stages of the cell cycle. Using a multicolor fluorescence in situ hybridization approach based on combinatorial probe labeling and digital imaging microscopy we demonstrate the simultaneous visualization of probe sets specific for chromosomes 13, 18, 21, X and Y. This approach enables one to evaluate aberrations of multiple chromosomes in a single hybridization experiment using metaphase chromosomes and interphase nuclei from a variety of cell types, including lymphocytes and amniocytes.