LINE-1 hypomethylation level as a potential prognostic factor for epithelial ovarian cancer
LINE-1 hypomethylation level as a potential prognostic factor for epithelial ovarian cancer
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DOI：
10.1111/j.1525-1438.2007.01117.x
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发表时间：
2008-07-01
影响因子：
4.8
通讯作者：
Mutirangura, A.
中科院分区：
文献类型：
--
作者：
Pattamadilok, J.;Huapai, N.;Mutirangura, A.
全基因组低甲基化是癌症中常见且至关重要的事件。本研究旨在评估常见上皮性卵巢癌（EOC）长散布元件1（LINE-1）重复序列甲基化水平在多期癌变过程中是否逐渐降低，以及LINE-1甲基化水平与临床病理特征之间的相关性。通过 COBRALINE-1（LINE-1 的组合亚硫酸氢盐限制性分析）PCR 方案检查了从 EOC 患者获得的总共 59 对显微解剖的 EOC 组织的 LINE-1 重复序列的甲基化水平。将甲基化水平与临床病理参数相关，以确定整体低甲基化作为 EOC 预后标志物的潜在作用。 59 个 EOC 的 LINE-1 甲基化水平（34.87 +/- 7.39%）低于代表性正常卵巢组织（46.89 +/- 8.31%；95% CI：9.42-14.62；P < 0.001，配对双尾 t 检验）。 LINE-1甲基化水平的降低与组织学亚型、较高的FIGO和晚期肿瘤分级相关。低甲基化程度较高的患者（即甲基化水平
A genome-wide hypomethylation is a common and crucial event in cancer. This study was to evaluate common epithelial ovarian cancer (EOC) if long interspersed element-1 (LINE-1) repetitive sequences methylation levels are progressively decreased during multistage carcinogenesis and there are the correlation between LINE-1 methylation levels and clinicopathologic characteristics. A total of 59 pairs of microdissected EOC tissues obtained from patients with EOC were examined for the methylation levels of LINE-1 repetitive sequences by a COBRALINE-1 (combined bisulfite restriction analysis of LINE-1) PCR protocol. The methylation levels were correlated with clinicopathologic parameters to determine the potential role of global hypomethylation as a prognostic marker for EOC. The LINE-1 methylation levels of 59 EOCs, 34.87 +/- 7.39%, were lower than in representative normal ovarian tissues (46.89 +/- 8.31%; 95% CI: 9.42-14.62; P < 0.001, paired-two-tailed t test). A decrease in the LINE-1 level of methylation was correlated with histological subtypes, higher FIGO and advanced tumor grade. Patients with greater hypomethylation (i.e., a methylation level