Culture of isolated human adipocytes and isolated adipose tissue.
Culture of isolated human adipocytes and isolated adipose tissue.
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DOI：
10.1007/978-1-61779-367-7_14
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Fried, Susan K
中科院分区：
文献类型：
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作者：
Carswell, Kirstin A;Lee, Mi-Jeong;Fried, Susan K
脂肪组织不再仅仅被认为是人体器官的隔热材料或填充物。它本身就是一个内分泌器官，包括具有分化为多个细胞系的能力的复合细胞。事实上，越来越多的证据支持这一理论，即肥胖及其相关代谢紊乱的原因源于细胞或组织水平。脂肪细胞功能障碍和慢性炎症状态能够直接通过细胞因子和脂肪因子的释放来调节甘油三酯的储存和动员。脂肪细胞分离和培养技术之间存在着显着的差异，这可能会影响实验结果。我们的目的是解释控制这些变量的重要性，以帮助针对研究结果进行方法选择，并相应地修改所使用的技术。本章描述的技术可产生细胞和脂肪组织，可用于许多不同的方式，包括用于分化的脂肪组织干细胞、DNA分析、RT-PCR、免疫组织化学、脂肪分解、葡萄糖摄取和LPL活性。
Adipose tissue (AT) is no longer considered merely as insulation or padding for human organs. It is an endocrine organ in its own right, which includes composite cells with the ability to differentiate into multiple cell lines. In fact, there is increasing evidence to support the theory that the causation of obesity and its associated metabolic disorders originate at the cellular or tissue level. Adipocyte dysfunction and chronic inflammatory states are able to modulate triglyceride storage and mobilization directly through cytokine and adipokine release. Significant variability exists between adipocyte isolation and culture techniques which subsequently can impact experimental results. We aim to explain the importance of controlling these variables, to assist tailoring methodological choice towards the investigational outcomes, and modifications of the techniques used accordingly. The techniques described in this chapter yield cell and adipose tissue which can be utilised in many different ways, including adipose tissue stem cells for differentiation, DNA analysis, RT-PCR, immunohistochemistry, lipolysis, glucose uptake, and LPL activity.