[An evaluation of blebs after filtering surgery with the in vivo confocal microscope].
[An evaluation of blebs after filtering surgery with the in vivo confocal microscope].
复制标题
[用体内共聚焦显微镜评估滤过手术后的气泡]。
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
C. Baudouin
中科院分区：
文献类型：
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作者：
A. Labbé;B. Dupas;P. Hamard;C. Baudouin
目的
用一种新的活体共聚焦显微镜描述滤过手术后的滤过泡。
方法
我们回顾性地评估了17名患者在小梁切除术或深层非穿透性巩膜切除术后的20个滤过泡。眼科检查包括裂隙灯检查、压平眼压计和体内共焦显微镜成像（Heidelberg Retina Tomograph II罗斯托克角膜模块）。将眼睛分为三组：滤过泡（8只眼），包裹或扁平的非滤过泡（7只眼）和丝裂霉素C滤过泡（5只眼）。在体内共聚焦显微镜图像进行了比较的形态和功能方面的滤过泡。
结果
滤过泡有大量上皮内微囊，而非滤过泡没有或很少。但在包裹性滤过泡中很少见到微囊。滤过泡结缔组织排列疏松，非滤过泡结缔组织致密。丝裂霉素C滤过泡内有大量微囊和疏松排列的上皮下结缔组织。
结论
通过在细胞水平上评价滤过泡，这种原始方法与离体组织学检查高度一致。在体内共聚焦显微镜构成了一个新的有前途的方式来了解伤口愈合机制的过滤手术。
PURPOSE
To describe blebs after filtering surgery with a new in vivo confocal microscope.
METHODS
We retrospectively evaluated 20 filtering blebs of 17 patients after trabeculectomy or deep nonpenetrating sclerectomy. Ophthalmological examinations included slit-lamp examination, applanation tonometry and in vivo confocal microscopy imaging (Heidelberg Retina Tomograph II Rostock Cornea Module). Eyes were classified into three groups: filtering blebs (eight eyes), nonfiltering blebs that were encapsulated or flat (seven eyes) and filtering blebs with mitomycin C (five eyes). In vivo confocal microscopy images were compared to the morphological and functional aspects of filtering blebs.
RESULTS
Filtering blebs had numerous intraepithelial microcysts, whereas nonfiltering blebs had none or few. However, few microcysts were seen in the encapsulated filtering blebs. The connective tissue of filtering blebs was arranged loosely, whereas it was dense in nonfiltering blebs. Filtering blebs with mitomycin C had numerous microcysts and loosely arranged subepithelial connective tissue.
CONCLUSION
By evaluating filtering blebs at the cellular level, this original method is highly consistent with ex vivo histological examination. In vivo confocal microscopy constitutes a new promising way to understand the wound healing mechanism in filtering surgery.