ASSOCIATION OF HUMAN CYCLIN-E WITH A PERIODIC G(1)-S PHASE PROTEIN-KINASE
ASSOCIATION OF HUMAN CYCLIN-E WITH A PERIODIC G(1)-S PHASE PROTEIN-KINASE
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DOI：
10.1126/science.1329201
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发表时间：
1992-09-25
期刊：
影响因子：
56.9
通讯作者：
REED, SI
中科院分区：
文献类型：
--
作者：
DULIC, V;LEES, E;REED, SI
G1 细胞周期蛋白控制芽殖酵母（酿酒酵母）的 G1 到 S 相转变。细胞周期蛋白 E 是在筛选人类互补 DNA 的过程中发现的，该 DNA 可以弥补芽殖酵母中 G1 细胞周期蛋白功能的缺陷。细胞周期蛋白 E 蛋白和相关蛋白激酶活性的数量在人类细胞周期中周期性波动；两者均在 G1 晚期和 S 期早期达到最大值。细胞周期蛋白 E 相关激酶活性与含有细胞周期蛋白 E 和细胞周期蛋白依赖性激酶 Cdk2 的复合物的出现相关。因此，细胞周期蛋白E-Cdk2复合物可能构成人G1-S期特异性调节蛋白激酶。
G1 cyclins control the G1 to S phase transition in the budding yeast, Saccharomyces cerevisiae. Cyclin E was discovered in the course of a screen for human complementary DNAs that rescue a deficiency of G1 cyclin function in budding yeast. The amounts of both the cyclin E protein and an associated protein kinase activity fluctuated periodically through the human cell cycle; both were maximal in late G1 and early S phases. Cyclin E-associated kinase activity was correlated with the appearance of complexes containing cyclin E and the cyclin-dependent kinase Cdk2. Thus, the cyclin E-Cdk2 complex may constitute a human G1-S phase-specific regulatory protein kinase.