Recombinant interleukin 4/BSF‐1 promotes growth and differentiation of intrathymic T cell precursors from fetal mice in vitro.
Recombinant interleukin 4/BSF‐1 promotes growth and differentiation of intrathymic T cell precursors from fetal mice in vitro.
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重组白细胞介素 4/BSF-1 在体外促进胎鼠胸腺内 T 细胞前体的生长和分化。
DOI：
10.1002/j.1460-2075.1987.tb04723.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
H. Boehmer
中科院分区：
文献类型：
--
作者：
R. Palacios;P. Sideras;H. Boehmer
重组小鼠白介素4/BSF-1(rIL4/BSF-1)联合佛波酯(PMA)可促进胎鼠(14-15天)胸腺内约四种T细胞前体细胞中的一种生长。即使是高浓度的抗白细胞介素2受体的单抗也不能抑制这种反应。经rIL4/BSF-1和PMA激活的胎儿胸腺细胞在培养7-21天后产生细胞溶解T细胞。免疫荧光染色和流式细胞仪分析显示，所有增殖细胞均为Thy1+，部分细胞表达Lyt2，但未检测到L3T4 T细胞分化抗原和细胞膜上T细胞抗原受体F23.1。结论：rIL4/BSF-1对正常胸腺内T细胞前体细胞具有生长和分化作用。这些结果为替代白介素2的生长因子参与T细胞前体细胞的增殖提供了证据。这些发现为研究正常胸腺内T细胞前体细胞在体外分化过程中的细胞和分子事件开辟了新的直接途径，并扩大了IL4/BSF-1靶细胞的谱。
Recombinant mouse interleukin 4/BSF‐1 (rIL4/BSF‐1) together with phorbol myristate acetate (PMA) promotes growth of one out of approximately four intrathymic T cell precursors from fetal mice (14‐15 days gestation). This response is not inhibited by even high concentrations of monoclonal antibody against the receptor for interleukin 2. Fetal thymocytes activated by rIL4/BSF‐1 plus PMA give rise to cytolytic T cells after 7‐21 days of culture. All the proliferating cells are Thy1+, some of them express Lyt2 but none has detectable L3T4 T cell differentiation antigens nor T cell antigen receptor (F23.1) on the cell membrane as assessed by immunofluorescence staining and flow fluorocytometry analysis. It is concluded that rIL4/BSF‐1 exerts both growth and differentiation activities on normal intrathymic T cell precursors. The results provide evidence for an alternative growth factor to interleukin 2 involved in proliferation of T cell precursors. These findings open new and direct ways of studying cellular and molecular events during the differentiation of normal intrathymic T cell precursors in vitro and extend the spectrum of target cells for IL4/BSF‐1.