Identification of brainstem interneurons projecting to the trigeminal motor nucleus and adjacent structures in the rabbit
Identification of brainstem interneurons projecting to the trigeminal motor nucleus and adjacent structures in the rabbit
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DOI：
10.1016/s0891-0618(00)00061-2
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发表时间：
2000-07-01
影响因子：
2.8
通讯作者：
Lund, JP
中科院分区：
文献类型：
--
作者：
Kolta, A;Westberg, KG;Lund, JP
内侧桥髓网状结构内的几个核的神经元在咀嚼过程中是活跃的，但它们与模式产生回路的其他元素的关系从未明确定义。在本文中，我们研究了该区域与三叉神经运动核和与侧脑干末级中间神经元池的联系。逆行示踪技术结合免疫组织化学用于确定谷氨酸能和gaba能神经元的群体。向第5运动核注射示踪剂后，可标记同侧中脑核、对侧第5运动核、主感觉核背帽和双侧脊核吻侧等三叉神经核中的神经元。许多末级中间神经元形成一条双侧侧带，从h区（围绕第v运动核的区域）向尾侧延伸，穿过细胞旁网状结构和第v脊髓尾侧核。将示踪剂注射到富含末阶中间神经元的区域h区，除了上面列出的区域外，还在双侧内侧网状结构中标记了大量神经元。注射部位之间的主要区别是，大多数投射到第v运动核的神经元位于外侧，而大多数投射到h区的神经元位于内侧。两个群体均含有谷氨酸能神经元和gaba能神经元。我们的研究结果表明，咀嚼过程中活跃的内侧网状结构神经元通过h区和其他邻近核的继电器影响Vth运动神经元的输出。(C) 2000 Elsevier Science B.V.版权所有
Neurons of several nuclei within the medial pontomedullar reticular formation are active during mastication, but their relationship with other elements of the pattern generating circuits have never been clearly defined. In this paper, we have studied the connection of this area with the trigeminal motor nucleus and with pools of last-order interneurons of the lateral brainstem. Retrograde tracing techniques were used in combination with immunohistochemistry to define populations of glutamatergic and GABAergic neurons. Injections of tracer into the Vth motor nucleus marked, neurons in several trigeminal nuclei including the ipsilateral mesencephalic nucleus, the contralateral Vth motor nucleus, the dorsal cap of the main sensory nucleus and the rostral divisions of the spinal nucleus bilaterally. Many last-order interneurons formed a bilateral lateral band running caudally from Regio h (the zone surrounding the Vth motor nucleus), through the parvocellular reticular formation and Vth spinal caudal nucleus. Injections of tracer into Regio h, an area rich in last-order interneurons, marked, in addition to the areas listed above, a large number of neurons in the medial reticular formation bilaterally. The major difference between injection sites was that most neurons projecting to the Vth motor nucleus were located laterally, whereas most of those projecting to Regio h were found medially. Both populations contained glutamatergic and GABAergic neurons intermingled. Our results indicate that neurons of the medial reticular formation that are active during mastication influence Vth motoneurons output via relays in Regio h and other adjacent nuclei. (C) 2000 Elsevier Science B.V. All rights reserved.