Characterization of the phylogenetic distribution and chromosomal insertion sites of five IS6110 elements in Mycobacterium tuberculosis: Non-random integration in the dnaA - dnaN region
Characterization of the phylogenetic distribution and chromosomal insertion sites of five IS6110 elements in Mycobacterium tuberculosis: Non-random integration in the dnaA - dnaN region
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DOI：
10.1054/tuld.1998.0003
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发表时间：
1998-01-01
期刊：
影响因子：
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通讯作者：
Kreiswirth, Barry N.
中科院分区：
文献类型：
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作者：
Kurepina, N. E.;Sreevatsan, S.;Kreiswirth, Barry N.
地点：耐多药结核分枝杆菌W株有5个IS6110染色体插入位点。目的：利用插入位点探针研究IS6110基因在结核分枝杆菌基因组中的系统发育分布。设计：用标准的IS6110 Southern杂交方法对722株结核分枝杆菌进行基因分型，再与A区插入位点探针杂交，代表性菌株再与B区和C区探针杂交。根据IS6110在这些不同区域的插入对菌株进行分组，并通过对IS6110插入位点的测序进一步比较它们的亲缘关系。结果：插入位点探针显示，以前归入北京毒株家族的中国分离株的IS6110插入序列与W和其他基因类型的1组毒株相同。出乎意料的是，我们发现IS6110在编码脱氧核糖核酸复制蛋白的dna A和dna基因之间整合了至少10个独立的时间。结论：IS6110插入点图能够识别代表物种多样性广度的结核分枝杆菌临床菌株集合之间的遗传关系。图谱数据表明，IS6110插入位点并不总是随机的。
Setting: Five IS6110 chromosomal insertion sites were characterized in the multi drug resistant Mycobacterium tuberculosis 'W' strain. Objective: To use insertion site probes to study the phylogenetic distribution of IS6110 in the M. tuberculosis genome. Design: A total of 722 M. tuberculosis isolates, previously genotyped using the standard IS6110 Southern blot hybridization methodology, were re-hybridized with the Region A insertion site probe and representative strains were further hybridized with the Region B and C probes. Strains were grouped on the basis of having IS6110 insertions in these different regions and their relatedness was further compared by sequencing the IS6110 insertion sites. Results: The insertion site probes revealed that the collection of Chinese isolates previously grouped as the Beijing strain family shared IS6110 insertions in common with the W and other genotypic group 1 strains. Unexpectedly, we found that IS6110 integrated at least 10 independent times between the dnaA and dnaN genes encoding deoxyribonucleic acid replication proteins. Conclusions: IS6110 insertion site mapping is able to identify genetic relatedness among a collection of M. tuberculosis clinical strains representing the breadth of species diversity. The mapping data indicate that IS6110 insertion sites are not always random.