Regulation of dome formation in kidney epithelial cell cultures.
Regulation of dome formation in kidney epithelial cell cultures.
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DOI：
10.1111/j.1749-6632.1981.tb15489.x
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发表时间：
1981-01-01
影响因子：
5.2
通讯作者：
Lever, J E
中科院分区：
文献类型：
--
作者：
Lever, J E
穹隆形成代表在某些上皮细胞培养物中维持的特化上皮特性的体外表现。当首次在乳腺上皮培养物中观察到圆顶时，以前认为是病毒转化细胞的病灶，但现在认为是细胞单层和培养皿之间液体积聚的病灶区域（示意图见图1，相差显微镜见图2）。这种特性并非乳腺上皮细胞所独有，而是在多种来源于转运上皮的上皮细胞类型中观察到的，L来源于这些细胞培养单层作为水室之间的渗透屏障和矢量泵的能力。最佳表征的上皮细胞系之一是MDCK肾上皮细胞系。5-14 MDCK细胞培养物的质膜紧密连接和结构极化成典型的顶端和基底侧表面的形态学证据表明，这些分化的特性在体外保留。6-11，13此外，MDCK肾上皮细胞培养物催化主动跨上皮流体转运，并表现出”渗漏”上皮的电阻和被动渗透性质。9，11，9，15，16，18，19，19，目前尚不清楚是否有任何生物化学或功能变化伴随着圆顶的形成。存在的可能性，圆顶可能代表功能的变化，而不是在质膜中的运输系统，如粘附到底层或在交界处的接触的变化。
Dome formation represents an in vitro manifestation of specialized epithelial properties maintained in certain epithelial cell cultures. Domes were formerly believed to be foci of virally transformed cells when first observed in mammary epithelial cultures, l but are now thought to represent focal regions of fluid accumulation between the cell monolayer and the culture dish (shown schematically in FIGURE 1 and by phase-contrast microscopy in FIGURE 2). This property, not unique to mammary epithelial cells, but observed in a wide variety of epithelial cell types derived from transporting epithelia,?-l derives from the ability of these cell-culture monolayers to act both as permeability barriers and vectorial pumps between aqueous compartments. One of the best characterized epithelial cell lines is the MDCK kidney epithelial cell line. 5-14 Morphological evidence for tight junctions and structural polarization of the plasma membrane of MDCK cell cultures into typical apical and basolateral surfaces indicates that these differentiated properties are retained in vitro. 6-11, l3 Furthermore, MDCK kidney epithelial cell cultures catalyze active transepithelial fluid transport and exhibit electrical resistance and passive permeation properties of" leaky" epithelia.', 9, 1 1 9 15Active fluid transport is required for dome formation, since addition of ouabain causes disappearance of domes. I6 It is not known whether any biochemical or functional changes accompany dome formation. The possibility exists that domes may represent changes in functions other than transport systems in the plasma membrane, such as changes in adhesion to the substratum or in junctional contacts.