Sustained attention performance deficits in the three-choice serial reaction time task in male and female rats after experimental brain trauma.
Sustained attention performance deficits in the three-choice serial reaction time task in male and female rats after experimental brain trauma.
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实验性脑损伤后雄性和雌性大鼠在三选择系列反应时间任务中持续的注意力表现缺陷。
DOI：
10.1016/j.brainres.2023.148336
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发表时间：
2023
期刊：
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
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作者：
Kutash,LindsayA;Moschonas,EleniH;O'Neil,DarikA;Craine,TimothyJ;Iouchmanov,AnnaL;Sunleaf,CarlsonR;Nicholas,MelissaA;Grobengieser,KatherineO;Patel,AartiK;Toader,Mihaela;Ranellone,TylerS;Rennerfeldt,PiperL;Cheng,JeffreyP;
对于许多创伤性脑损伤（TBI）幸存者来说，注意力受损是与长期后遗症相关的认知缺陷的核心。评估创伤性脑损伤后的复杂持续注意力具有临床相关性，并可能为制定男性和女性的治疗和康复目标提供可靠的途径。我们假设，遭受中度脑外伤的大鼠在操作性3-选择系列反应时间任务（3-CSRT）中表现出注意力缺陷，即准确性降低和注意力分散增加，该任务设计为临床连续表现测试的类似物。在300 ms提示达到70%准确度的基线后，成年雄性和雌性Sprague-Dawley大鼠在右侧顶叶皮层受到控制的皮质冲击（以4 m/s的速度变形2.8 mm）或假损伤。恢复两周后，再次进行为期10天的3-CSRT测试。相关测量包括准确率百分比（总体和三个线索端口中的每一个），遗漏百分比，以及工具戳和检索奖励的延迟。结果表明，与Sham大鼠和他们自己的侮辱前基线相比，在tbi后重新测试3-CSRT时，雄性和雌性都显示出降低的准确率和增加的遗漏（p < 0.05）。在术后10天的重新测试中，表现准确性持续受损，表明持续注意力过程存在明显且持久的功能障碍。当提示伪随机出现在左侧提示端口时，缺陷特别明显（p< 0.05），反映了临床半半球忽视。这些数据表明，创伤性脑损伤后男女均存在显著且持续的复杂注意力障碍，因此确定有效的认知恢复疗法至关重要。
Impaired attention is central to the cognitive deficits associated with long-term sequelae for many traumatic brain injury (TBI) survivors. Assessing complex sustained attention post-TBI is clinically-relevant and may provide reliable avenues towards developing therapeutic and rehabilitation targets in both males and females. We hypothesized that rats subjected to a moderate TBI will exhibit attentional deficits seen as reduced accuracy and increased distractibility in an operant 3-choice serial reaction time task (3-CSRT), designed as an analogue of the clinical continuous performance test. Upon reaching baseline of 70% accuracy at the 300 ms cue, adult male and female Sprague-Dawley rats were subjected to a controlled cortical impact (2.8 mm deformation at 4 m/s) or sham injury over the right parietal cortex. After two weeks of recovery, they were retested on the 3-CSRT for ten days. Dependent measures include percent accuracy (overall and for each of the three cue ports), percent omissions, as well as latency to instrumental poke and retrieve reward. Results demonstrate that both males and females displayed reduced percent accuracy and increased omissions when re-tested post-TBI on 3-CSRT compared to Sham rats and to their own pre-insult baseline (p’s < 0.05). Performance accuracy was impaired consistently throughout the ten days of post-surgery re-testing, suggesting pronounced and long-lasting dysfunction in sustained attention processes. Deficits were specifically more pronounced when the cue was pseudorandomly presented in the left-side cue port (p< 0.05), mirroring clinical hemispatial neglect. These data demonstrate significant and persistent complex attention impairments in both sexes after TBI, rendering identifying efficient therapies for cognitive recovery as pivotal.