INTERACTIONS BETWEEN SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES IN FORMATION OF SPLICEOSOMES
INTERACTIONS BETWEEN SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES IN FORMATION OF SPLICEOSOMES
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DOI：
10.1016/0092-8674(87)90614-3
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发表时间：
1987-06-19
期刊：
影响因子：
64.5
通讯作者：
SHARP, PA
中科院分区：
文献类型：
--
作者：
KONARSKA, MM;SHARP, PA
在非变性凝胶中电泳分离核糖核蛋白颗粒用于分析mRNA前体的剪接。在反应的早期，形成了由U2小核核糖核蛋白颗粒（snRNP）结合到3“剪接位点上游序列组成的复合物。该复合物被建模为更大复合物的前体，剪接体，其包含U2，U4/6和U 5 snRNP。U2 snRNP-前体RNA复合物向剪接体的转化可能涉及含有U4/6和U 5 snRNP的单个多snRNP颗粒的结合。切除的内含子被释放在一个复合物中，该复合物含有U 5、U6，可能还有U2 snRNP。令人惊讶的是，U4 snRNP不是含内含子复合物的一部分，这表明U4/6 snRNP在剪接过程中分解和组装。随后，重组的U4/6 snRNP将与U 5 snRNP结合并参与从头剪接体的形成。在任何剪接复合物中均未检测到U1 snRNP。
Electrophoretic separation of ribonucleoprotein particles in a nondenaturing gel was used to analyze the splicing of mRNA precursors. Early in the reaction, a complex formed consisting of the U2 small nuclear ribonucleoprotein particle (snRNP) bound to sequences upstream of the 3'' splice site. This complex is modeled as a precursor of a larger complex, the spliceosome, which contains U2,U4/6, and U5 snRNPs. Conversion of the U2 snRNP-precursor RNA complex to the spliceosome probably involves binding of a single multi-snRNP particle containing U4/6 and U5 snRNPs. The excised intron was released in a complex containing U5, U6, and probably U2 snRNPs. Surprisingly, U4 snRNP was not part of the intron-containing complex, suggesting that U4/6 snRNP disassembles and assembles during splicing. Subsequently, the reassembled U4/6 snRNP would associate with U5 snRNP and participate in de novo spliceosome formation. U1 snRNP was not detected in any of the splicing complexes.