Origin, distribution and mapping of RAPD markers from wildPetunia species inPetunia hybrida Hort lines
Origin, distribution and mapping of RAPD markers from wildPetunia species inPetunia hybrida Hort lines
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野生矮牵牛品种 RAPD 标记的起源、分布和作图
DOI：
10.1007/bf01253965
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发表时间：
1994
影响因子：
5.4
通讯作者：
André Bervillé
中科院分区：
文献类型：
--
作者：
D. Peltier;E. Farcy;Hubert Dulieu;André Bervillé
本研究建立了第一张基于RAPD和表型标记的矮牵牛遗传连锁图谱。研究的后代由100个来自[（St40xTlvl）xTlvl]回交的BC1个体组成。每个形态学标记先前都被映射到七条染色体中的一条上。该图谱由35个RAPD位点组成，其中24个位点受染色体影响，10个位点未受影响。该位点的覆盖范围为262.9cM，平均距离为8.2cM。它们分布在七个连锁群上，其中六个在已识别的染色体上。还将RAPD标记应用于一组tenP。杂交种，线选择的多样性和一套7个野生物种对应的可能祖先的P。杂交种这些标记在野生种和野生种中都有发现。杂交系表明它们是遗传的，并且足够稳定，可以建立相似性，并表明物种之间的关系。10个品系中有8个品系携带不同的RAPD标记连锁群，这表明起源于野生种的染色体之间发生了重组事件。
We have established the first linkage map forPetunia hybridabased upon both RAPD and phenotypical markers. The progeny studied consisted of 100 BC1 individuals derived from the [(St40xTlvl)xTlvl] back-cross. Each morphological marker has previously been mapped onto one of the seven chromosomes. The map consists of 35 RAPD loci of which 24 were affected onto chromosomes while 10 loci were not affected. The loci covered 262.9 cM with a mean distance of 8.2 cM. They are dispersed over seven linkage groups, of which six are carried on identified chromosomes. The RAPD markers were also applied on a set of tenP. hybrida, lines chosen for their diversity and on a set of seven wild species corresponding to the possible ancestors of theP. hybridaspecies. The markers were found both in the wild species as well as inP. hybridalines indicating that they are inherited and are stable enough to establish similarities and to suggest relationships between species. Eight out of the ten lines carry different linkage groups of RAPD markers, which suggest that recombinant events occurred between chromosomes which originated in the wild species.