Construction and characterization of an infectious molecular clone of novel duck reovirus.
Construction and characterization of an infectious molecular clone of novel duck reovirus.
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DOI：
10.1099/jgv.0.001036
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发表时间：
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期刊：
影响因子：
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通讯作者：
Zongyan Chen
中科院分区：
文献类型：
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作者：
Qiaomei Wu;Ming Ding;Guangqing Liu;Zongyan Chen
Novel Duck reovirus (NDRV), the prototype strain of species Avian orthoreoviruses (ARVs) is currently an infectious agent for ducks. Studies on NDRV replication and pathogenesis have been hampered by the lack of an available reverse genetics system. In this study, a plasmid-based reverse genetics system free of helper viruses has been developed. In this system, 10 full-length gene segments of wild-type NDRV TH11 strain are transfected into BSR-T7/5 cells which express bacteriophage T7 RNA polymerase. Production of infectious virus was shown by inoculation of cell lysate derived from transfected cells into 10-day duck embryos. The growth kinetics and infectivity in vivo of recombinant strains were identical to those of wild-type strain. These viruses grew well and were genetically stable both in vitro and in vivo. Altogether, these results show the successful production of an infectious clone for NDRV. The infectious clone reported will be further used to elucidate the mechanism of host tropism, viral replication and pathogenesis as well as immunological changes induced by NDRV.
