The chitinase gene (Bbchit1) from Beauveria bassiana enhances resistance to Cytospora chrysosperma in Populus tomentosa Carr
The chitinase gene (Bbchit1) from Beauveria bassiana enhances resistance to Cytospora chrysosperma in Populus tomentosa Carr
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来自白僵菌的几丁质酶基因 (Bbchit1) 增强了毛白杨对金子孢子虫的抗性。
DOI：
10.1007/s10529-010-0297-6
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发表时间：
2010-09-01
影响因子：
2.7
通讯作者：
Wang, Zhuanhua
中科院分区：
文献类型：
--
作者：
Ren, Xiaoxia;Zhang, Xin;Wang, Zhuanhua
毛白色白杨（Populus tomentosa卡尔）易受植物病害感染，严重影响其生长并显著降低其经济价值。将球孢白僵菌几丁质酶基因Bbchit 1导入白色（Populus tomentosa卡尔.）农杆菌介导的转化。植物转化载体的T-DNA含有CaMV 35 S启动子控制的β-葡萄糖醛酸酶报告基因（GUS）和nos启动子控制的新霉素磷酸转移酶选择标记基因（NPTII）。在大多数卡那霉素抗性植株中检测到GUS活性。使用PCR证实转基因在植物基因组中的稳定整合。RT-PCR分析表明，Bbchit 1基因在转化植株中得到了转录。用离体测定法评价了转基因株系对白杨病原真菌的抗性，结果表明，转基因株系的叶片和枝条粗提物对病原真菌Cytospora chrysosperma（E.A.）类似地，Bbchit 1过表达增强了对C.结果表明，该基因在杨树抗真菌方面具有潜在的应用价值。
The Chinese white poplar (Populus tomentosa Carr.) is susceptible to infection by plant diseases which severely affect its growth and substantially decrease its economic value. A chitinase gene (Bbchit1) from Beauveria bassiana was introduced into Chinese white poplar (Populus tomentosa Carr.) by Agrobacterium-mediated transformation. The T-DNA of plant transformation vector contained the beta-glucuronidase reporter gene (GUS) under the control of CaMV 35S promoter and the neomycin phosphotransferase selection marker gene (NPTII) driven by the nos promoter. GUS activity was detected in most of the kanamycin-resistant plants tested. Stable integration of transgenes in the plant genome was confirmed using PCR. RT-PCR analysis showed that the Bbchit1 gene was transcribed in the transformed plants. When evaluated for resistance to poplar fungal pathogens with an in vitro assay, crude extracts from leaves and shoots of transgenic lines were inhibitory against the pathogenic fungus Cytospora chrysosperma (Pers.) Fr. Similarly, Bbchit1 overexpression enhanced disease resistance to C. chrysosperma in the transformed poplar plants, indicating that is gene is potentially useful to protect the trees against fungal diseases.