The novel long noncoding RNA lncRNA-Adi regulates adipogenesis
The novel long noncoding RNA lncRNA-Adi regulates adipogenesis
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新型长非编码RNA lncRNA-Adi调节脂肪生成
DOI：
10.1002/sctm.19-0438
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发表时间：
2020-05-01
影响因子：
6
通讯作者：
Liu, Lei
中科院分区：
文献类型：
--
作者：
Chen, Yuanwei;Li, Kaide;Liu, Lei
脂肪生成参与了肥胖、糖尿病等多种生理和病理过程，并受到一系列精确的分子事件调控。然而，参与这种调节的分子尚未完全表征。在这项研究中，我们确定了一个长的非编码（lnc）RNA，lncRNA-Adi，这是高度表达的脂肪组织来源的基质细胞（ADSC）分化成脂肪细胞。lncRNA-Adi的敲低可抑制ADSCs的成脂分化能力。此外，发现lncRNA-Adi与microRNA（miR）-449a相互作用，以增强脂肪形成过程中细胞周期蛋白依赖性激酶（CDK）6的表达。lncRNA-Adi调节脂肪形成的机制被确定为涉及lncRNA-Adi-miR-449 a相互作用，其与CDK 6 3 '非翻译区竞争以增加CDK 6翻译并激活pRb-E2 F1途径以促进脂肪形成。这些发现为寻找肥胖和糖尿病等代谢紊乱的治疗靶点提供了有价值的信息和新的研究角度。
Adipogenesis participates in many physiological and pathological processes such as obesity and diabetes, and is regulated by a series of precise molecular events. However, the molecules involved in this regulation have not been fully characterized. In this study, we identified a long noncoding (lnc)RNA, lncRNA-Adi, which is highly expressed in adipose tissue-derived stromal cells (ADSCs) that are differentiating into adipocytes. Knockdown of lncRNA-Adi impaired the adipogenic differentiation ability of ADSCs. Moreover, lncRNA-Adi was found to interact with microRNA (miR)-449a to enhance the expression of cyclin-dependent kinase (CDK)6 during adipogenesis. The mechanism by which lncRNA-Adi regulates adipogenesis was determined to involve an lncRNA-Adi-miR-449a interaction that competes with the CDK6 3 ' untranslated region to increase CDK6 translation and activate the pRb-E2F1 pathway to promote adipogenesis. These findings provide valuable information and a new study angle to search for therapeutic targets against metabolic disorders such as obesity and diabetes.